# REPLICATIVE MECHANISMS OF THE NEGATIVE STRAND VIRUSES

> **NIH NIH R01** · DUKE UNIVERSITY · 1991 · $208,242

## Abstract

Acute viral infections are usually associated with cell killing in
that major cellular functions may be inhibited or destroyed in the
Process of replication of the virus.  Acute viruses of the negative
strand RNA group include measles, mumps, rabies, respiratory
syncytial virus and the model prototype for molecular studies,
vesicular stomatitis virus (VSV).  The objectives of this proposal
are to understand the molecular nature of virus-host interactions
that lead to the cytopathology associated with infection by VSV.
These experiments will focus on nucleic acid-protein interactions
that are thought to affect host cell macromolecular events such as
transcription and RNA processing.  We will use antibody
precipitation, recombinant DNA methods, cell culture techniques and
in vitro biochemical analysis.  Our goals are to define at the
molecular level those RNA-protein contacts that alter cellular
functions in order to understand mechanisms of: 1) cell killing by
VSV:  2) conversion from acute to persistent infection: 3) cellular
RNA transcription and processing.  Thus, we are interested in VSV
as a biological entity with unique properties of replication and
pathology and as a probe to study the functions of mammalian cells.

## Key facts

- **NIH application ID:** 3126558
- **Project number:** 5R01AI016099-12
- **Recipient organization:** DUKE UNIVERSITY
- **SAM.gov UEI:** TP7EK8DZV6N5
- **Principal Investigator:** JACK D KEENE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $208,242
- **Award type:** 5
- **Project period:** 1979-08-01 → 1993-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126558

## Citation

> US National Institutes of Health, RePORTER application 3126558, REPLICATIVE MECHANISMS OF THE NEGATIVE STRAND VIRUSES (5R01AI016099-12). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126558. Licensed CC0.

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