# PROPERTIES OF FUNCTIONAL MACROPHAGE CELL LINES

> **NIH NIH R01** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 1989 · $276,731

## Abstract

The major goal of this proposal is to increase our understanding of the
molecular basis of macrophage function in three facets of the immune
response:

1) the activation of T helper cells.  We plan to isolate and characterize
variant progeny of an antigen presenting murine macrophage line that have
structural alterations in the I-E molecule.  Variants will be analyzed at
the protein and the DNA level.  These studies should help elucidate
structure-function relationships in Ia molecules.

2) the induction of T suppressor cells.  We are interested in identifying
and characterizing the membrane antigen on murine macrophages that
interacts wit I-J bearing T lymphocytes.  Macrophage lines will be
generated that bear this molecule.

3)  the interaction with antigen antibody complexes.  The interaction of
the immunoglobulin with murine macrophage Fc receptors will be further
analyzed and the requirements for binding of immunoglobulin and for
triggering of phagocytosis determined.  The specificity of these receptors
will be compared to those on B and T lymphocytes.  For these studies mutant
myeloma proteins and cleavage fragments of myeloma proteins will be used.

In addition we will use monocyte cell lines generated from peripheral blood
monocytes of normal individuals and patients with SLE to study Fc receptor
function on human monocytes.  We will also try to use human monocyte lines
as accessory cells in antigen presentation assays and isolate mutants in
human Class II histocompatibility antigens.

## Key facts

- **NIH application ID:** 3126591
- **Project number:** 5R01AI016166-11
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** Betty Diamond
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $276,731
- **Award type:** 5
- **Project period:** 1979-08-01 → 1991-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126591

## Citation

> US National Institutes of Health, RePORTER application 3126591, PROPERTIES OF FUNCTIONAL MACROPHAGE CELL LINES (5R01AI016166-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126591. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*