# CLONING AND EXPRESSION OF INFLUENZA VIRAL RNA SEGMENTS

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1988 · $267,996

## Abstract

Influenza viruses bud from the apical surface of polarized
epithelial cells.  The mechanism by which the budding is
restricted to the apical surface remains unknown.  We have shown
that influenza viral envelope glycoproteins either hemagglutinin
(HA) or neuraminidase (NA) when expressed from cloned cDNAs
are also transported to the apical surface in absence of other viral
proteins.  Our goal is to define the structural features ("signals")
of HA and NA involved in transport, sorting and polarized
expression.  We wll continue studying the function of different
domains of HA and NA and make specific alterations in the
nucleotide sequence of the insert DNA to achieve the desired
changes in the amino acid sequence of the polypeptides.  These
altered cDNA clones will be expressed and the fate of these
altered proteins will be followed.  Thus, we hope to determine the
role of the specific domains (or amino acid sequences) of HA and
NA in expression, sorting, and transport as well as in the biological
functions of these two important glycoproteins.  We plan to
continue using eukaryotic expression vectors to express these
proteins in animal cells.  We are using site-specific mutations,
deletions, as well as chimeric fusions between different genes to
achieve these objectives.  In addition, we are expressing influenza
viral HA and NA in yeast (Saccharomyces cerevisiae), which may
be of potential use in the development of an improved subunit
vaccine.

## Key facts

- **NIH application ID:** 3126627
- **Project number:** 2R01AI016348-09
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **SAM.gov UEI:** RN64EPNH8JC6
- **Principal Investigator:** DEBI P. NAYAK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $267,996
- **Award type:** 2
- **Project period:** 1980-05-01 → 1993-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126627

## Citation

> US National Institutes of Health, RePORTER application 3126627, CLONING AND EXPRESSION OF INFLUENZA VIRAL RNA SEGMENTS (2R01AI016348-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126627. Licensed CC0.

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