# TRANSEPITHELIAL MIGRATION OF HUMAN NEUTROPHILS

> **NIH NIH R01** · SUNY DOWNSTATE MEDICAL CENTER · 1987 · $87,683

## Abstract

The process of inflammation involves hemodynamic changes, increases in
vascular permeability and the accumulation of leukocytes in various organs
of the body.  To simplify a very complex series of interactions we have
devised two in vitro models of inflammation which enable us to eliminate
serum and connective tissue components and determine the direct effects of
various inflammatory mediators on the endothelium or epithelium itself or
on the process of neutrophil and/or monocyte transendothelial/
transepithelial migration.  The systems involve growing monolayers of
either human umbilical vein endothelial cells on the connective tissue of
amnion or kidney epithelial cells on micropore filters.  These systems
produce a transepithelial electrical resistance as high as 78 and 500
ohms.square cm, respectively.  The effect of 1) leukocyte (neutrophil and
mononuclear phagocyte), 2) chemoattractant (C5a and leukotriene B4), and/or
3) permeability factors (leukotriene C4&D4,C3a, platelet-activating factor,
and prostaglandin E2) on the disassembly, reassembly and permeability of
the endothelial and epithelial occluding junctions will be determined.
This will be examined by continuous transepithelial electrical resistance
studies, ultrastructural or radioactive tracer studies and freeze-fracture
electron microscopy.  The role of protein synthesis on the reassembly of
the occluding junction will be studied.  The effect of various
chemo-attractants and permeability factors on arachidonic acid metabolism
in human umbilical vein endothelial cells in situ as well as those grown in
culture will be determined.  Release of radiolabel from endothelial cells
prelabeled with 3H-arachidonic acid will provide a quantitative measure of
the release of arachidonic acid-derived products and a means to determine
by reverse-phase HPLC the arachidonic acid metabolites synthesized in
response to various stimuli.  In addition, differences in the rate, ability
to invade as well as possible interactions between monocytes and
neutrophils during the transendothelial/epithelialmigratory process will be
determined.  The changes and interactions of these phagocytes as they
remain in the connective tissue and the subsequent ability to leave the
connective tissue will be examined.  The results of these studies should
provide insight into the inflammatory response and should increase our
abilities to foster its beneficial effects and to limit its destructive
sequelae.

## Key facts

- **NIH application ID:** 3126674
- **Project number:** 5R01AI016480-06
- **Recipient organization:** SUNY DOWNSTATE MEDICAL CENTER
- **SAM.gov UEI:** NJ14V2NZYM68
- **Principal Investigator:** EVA B CRAMER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $87,683
- **Award type:** 5
- **Project period:** 1980-02-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126674

## Citation

> US National Institutes of Health, RePORTER application 3126674, TRANSEPITHELIAL MIGRATION OF HUMAN NEUTROPHILS (5R01AI016480-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126674. Licensed CC0.

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