# ESTABLISHMENT OF A GENETIC SYSTEM IN CANDIDA ABLICANS

> **NIH NIH R01** · MICHIGAN STATE UNIVERSITY · 1987 · $136,852

## Abstract

The object of the research is to devise a method of genetic analysis for
Candida albicans.  Previous work has shown that the cells are diploid, that
many natural isolates contain recessive auxotrophic mutations, and that
these mutations can be exposed by UV- and nitrous acid-induced mitotic
crossing over.  Spheroplast fusion can also be used to construct novel
phenotypes and identify alleles.  We will continue our mapping and
complementation studies using mitotic crossing-over and spheroplast
fusion.  We will also use a C. albicans clone bank to devise a
transformation system, based on the techniques used in Saccharomyces.  The
clone bank has been constructed in the chimeric E. coli-S. cerevisiae
vector YEp13 and consists of 4463 colonies, each containing about 7.5 kb of
Canadida DNA.  We will use this bank to look for C. albicans sequences
which complement S. cerevisiae nutritional deficiencies and for C. albicans
sequences which complement C. albicans auxotrophies.  We will also try to
use known S. cerevisiae genes( ARG4, TRP1, TRP5, H1S3) to complement C.
albicans deficiencies.  We will isolate mutants in properties of C.
albicans thought to be associated with virulence and test the mutants for
their virulence in mice.  These studies will further our knowledge of the
genetic basis of virulence in this important human pathogen and may serve
as a model system for parasexual analysis of other pathogens which are
presently recalcitrant to genetic investigations.

## Key facts

- **NIH application ID:** 3126713
- **Project number:** 5R01AI016567-08
- **Recipient organization:** MICHIGAN STATE UNIVERSITY
- **SAM.gov UEI:** R28EKN92ZTZ9
- **Principal Investigator:** LOREN SNYDER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $136,852
- **Award type:** 5
- **Project period:** 1980-06-01 → 1988-09-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126713

## Citation

> US National Institutes of Health, RePORTER application 3126713, ESTABLISHMENT OF A GENETIC SYSTEM IN CANDIDA ABLICANS (5R01AI016567-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126713. Licensed CC0.

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