# GENETIC CONTROL OF IMMUNOGLOBULIN VARIABLE REGIONS

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT CHICAGO · 1987 · $156,501

## Abstract

The purpose of the research outlined in this proposal is to examine the fine
structure of the chromosomal region which encodes variable regions of
immunoglobulin heavy chains.  Monoclonal antibodies (Mab's) against individual
rabbit VH allotypic specificities will be made in mouse-mouse lymphocyte
hybridomas.  Individual Mab's and mixtures of these Mab's will be reacted in a
quantitative radioprecipitation assay against rabbit IgG to identify
subpopulations of the VHa allotypes.  Each of the VH sub-populations identified
in individual rabbits will presumably reflect a different VH germ lines gene.
The molecular and cellular distribution of the various subpopulatins will be
examined in each of several rabbits; we will particularly look for VH a
subpopulations which are found in some but not all rabbits of that particular VH
a allotype.  The distribution of the VH a subpopulations in IgA "recombinant"
molecules will also be determined.

Other studies will be to test for cross-reaction of anti-al Mab's with IgG of
the a2 and a3 allotype as well as with human IgG.  These cross-reactions should
shed light on the evolution of VH genes.
Attempts will be made to prepare MAb to the allelic products of the VH genes x32
and y33 i.e., x minus and y minus.

## Key facts

- **NIH application ID:** 3126730
- **Project number:** 5R01AI016611-08
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **SAM.gov UEI:** W8XEAJDKMXH3
- **Principal Investigator:** Katherine L. Knight
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $156,501
- **Award type:** 5
- **Project period:** 1980-01-01 → 1987-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126730

## Citation

> US National Institutes of Health, RePORTER application 3126730, GENETIC CONTROL OF IMMUNOGLOBULIN VARIABLE REGIONS (5R01AI016611-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126730. Licensed CC0.

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