# TRANSCRIPTIONAL CONTROL OF GENE EXPRESSION

> **NIH NIH R01** · WASHINGTON STATE UNIVERSITY · 1987 · $166,388

## Abstract

The objective of this work is to further our understanding of the molecular
components and mechanisms involved in the regulation of gene expression.  This
proposal is based on our studies which have lead to the identification of the
transcriptional control elements that regulate inducible tetracycline resistance
in Escherichia coli K12.  We have localized the gene for a specific repressor
function and determined the DNA sequence of a region containing multiple
promoter-operator sites for the resistance genes.  Our objectives now are to:
1)  examine in greater detail the primary structure and function of these
regulatory elements, and 2)  define the overall gene structure and organization
of the resistance determinant.  We will test a model, consistent with our
current data, that transcription of the repressor gene and transcription of the
resistance gene(s) are initiated at divergent and functionally overlapping
promoter-operator sites, and that the repressor gene is subject to negative
autogenous regulation.  We will make extensive use of gene fusions between the
regulatory elements of the resistance genes and the lacZ structural gene in
order to isolate and characterize regulatory mutations.  DNA and RNA sequencing,
recombinant DNA, and in vitro transcription methodologies will be employed in
these investigations.

## Key facts

- **NIH application ID:** 3126806
- **Project number:** 7R01AI016735-08
- **Recipient organization:** WASHINGTON STATE UNIVERSITY
- **SAM.gov UEI:** XRJSGX384TD6
- **Principal Investigator:** KEVIN P BERTRAND
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $166,388
- **Award type:** 7
- **Project period:** 1987-03-01 → 1990-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126806

## Citation

> US National Institutes of Health, RePORTER application 3126806, TRANSCRIPTIONAL CONTROL OF GENE EXPRESSION (7R01AI016735-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126806. Licensed CC0.

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