# EFFECTORS IN THE ALLOGRAFT RESPONSE

> **NIH NIH R01** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 1988 · $204,934

## Abstract

The aim of this grant proposal is to determine under what conditions
delayed type hypersensitivity (DTH) reactions play a role in the rejection
of allografts.  Three criteria which define the capacity of a sensitized
lymphocyte in contact with its specific alloantigen to elicit a DTH have
been postulated: (1) the capacity to transfer delayed footpad swelling in a
syngeneic host, (2) the capacity to recruit circulating radio-labelled
lymphocytes, increase regional blood flow and alter vascular permeability,
and (3) the capacity to induce naive macrophages to alter function or
injure tissue in vitro.  Assays for each of these components of DTH
reactions have been established.  Carefully characterized subpopulations of
cells from sponge matrix allografts across defined histocompatibility
barriers (Class 1, Class 2, combinations of Class 1 and 2, minor antigenic
loci) will be assayed for their capacity to satisfy DTH criteria.  Only a
single recipient strain of mouse (B10.AQR) will be used so that the
comparisons between cell populations can be standardized for all genetic
disparities.  Alloantigen sensitizied cloned T cells isolated frm sponge
matrix allografts with well characterized helper or cytotoxic properties
will be compared in their DTH ability with subpopulations from the graft.
Particular attention will be focused on clones with helper function
responding to class 2 alloantigens.  We expect to be able to correlate
capacity of a coned T cell population to elicit the various DTH phenomena
wih its antigenic specificity, its surface marker and functional phenotype,
and with its capacity to secrete lymphokines in vitro.  We hope to
elucidate the mechanisms of tissue destruction during graft rejection in
the total absence of cytotoxic T cells such as that seen during the
rejection of grafts disparate only at the class 2 loci.

## Key facts

- **NIH application ID:** 3126870
- **Project number:** 5R01AI016869-09
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **SAM.gov UEI:** MKAGLD59JRL1
- **Principal Investigator:** RICHARD L. SIMMONS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $204,934
- **Award type:** 5
- **Project period:** 1987-08-01 → 1990-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126870

## Citation

> US National Institutes of Health, RePORTER application 3126870, EFFECTORS IN THE ALLOGRAFT RESPONSE (5R01AI016869-09). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3126870. Licensed CC0.

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