# PRODUCTION AND ANALYSIS OF HISTOCOMPATIBILITY MUTANTS

> **NIH NIH R01** · NORTHWESTERN UNIVERSITY · 1987 · $228,246

## Abstract

This project seeks to maintain, to develop and to expand a colony of mice
which carry mutations of genes which affect skin graft transplantation.  A
substantial number of lines are already on hand, completed or under
development.  These include strains bearing H-2 mutations as well as
non-H-2 gene mutations.  New mutations will be sought by screening the
progeny of ENU-treated or untreated male mice of strains C57BL/6Kh and
BALB/cKh.  In addition a number of other inbred, congenic and recombinant
strains will be continually screened as part of a system to monitor their
genetic integrity and mutants may be recovered from them as well.

Mutants will be entered into immunogenetic analyses, both as part of formal
collaborative efforts and by making them available to other investigators
upon request.  The formal collaborations include Dr. Stanley Nathenson
(biochemical and structural analysis of mutant gene products at the protein
and DNA levels), Dr. Ian McKenzie (serological analyses of mutant gene
products), Dr. Kees Melief (functional consequences of the mutations for
cell-mediated assays such as CML, MLR and MHC restriction), Dr. Peter
Wettstein (looking for changes in integrated viral sequences as a result of
mutations involving mutations of non-H-2 genes), and Dr. Byung Kim
(assisting in electrophoretic techniques).

We will be specifically examining the recently reovered H-2dm6 mutation
(which has lost Dd, but retained Ld and apparently Rd as well) to see if D
region products other than D,L and R can be identified which are missing in
the mutant.  We also propose attempting to raise alloantisera against some
of the minor histocompatibility mutants as a step toward future analyses of
these genes.

## Key facts

- **NIH application ID:** 3126897
- **Project number:** 5R01AI016919-09
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **SAM.gov UEI:** KG76WYENL5K1
- **Principal Investigator:** ROGER W MELVOLD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $228,246
- **Award type:** 5
- **Project period:** 1979-09-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126897

## Citation

> US National Institutes of Health, RePORTER application 3126897, PRODUCTION AND ANALYSIS OF HISTOCOMPATIBILITY MUTANTS (5R01AI016919-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126897. Licensed CC0.

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