# TRANSCRIPTION AND TRANSLATION REGULATION IN INFECTION

> **NIH NIH R01** · UNIVERSITY OF KANSAS LAWRENCE · 1987 · $88,244

## Abstract

This proposal seeks to continue identificstion and roles of factors
responsible for the pathobiological events in Q fever.  We have previously
demonstrated several of these in guinea pigs or L-cells infected with
Coxiella burnetii, the rickettsioid agent of Q fever (Fig. 1).  Prominent
in the pathobiology of infected guinea pig liver are:  stimulated
synythesis of RNA and protein, coincident with increased phosphorylation of
non-histone chromatin protein (NHCP) and ribosomal protein, increased
nuclear phosphoprotein kinase activity, and elevated levels of cortisol,
cyclic AMP and polyamines.  We propose a model (Fig. 2) in which levels of
cAMP, polyamines, cortisol and glucagon increase in response to infection,
triggering a series of interdependent reactions resulting in stimulated RNA
and protein synthesis, and other previously demonstrated features of Q
fever pathobiology.  We plan to extend these findings:  1.  to the
identification and role of spermine-dependent and -independent, and cyclic
mononucletide-dependent and -independent cytoplasmic and nuclear
phoshoprotein kinases whose activities increase by infection; 2.  to study
interrelations of phosphatase and protein kinase mediated reactions as
related to phosphorylation of NHCP and ribosmal protein in
infection-stimulated synthesis of RNA and protein; 3.  to determine what,
if any, is the significance of ribosomal protein phosphorylation induced by
infection and the role in stimulated translation; 4.  to resume previous
studies on the effects of C. burnetii lipopolysaccharide (LPS) on guinea
pigs and cultured cells.  (Our laboratory was first to isolate and
characterize this LPS); 5.  to see whether infection and/or LPSs imulates
glucagon secretion, suggested by observed glycogenolysis, inhibited
glucogen synthase, and stimulated phosphorylase and lipase activities.

Elucidating the nature and regulation of pathobiological change induced by
the Q fever models may aid in clarifying similar events in other types of
infectious and non-infectious diseases.

## Key facts

- **NIH application ID:** 3126937
- **Project number:** 5R01AI016954-06
- **Recipient organization:** UNIVERSITY OF KANSAS LAWRENCE
- **SAM.gov UEI:** SSUJB3GSH8A5
- **Principal Investigator:** DAVID PARETSKY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $88,244
- **Award type:** 5
- **Project period:** 1981-08-01 → 1988-10-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3126937

## Citation

> US National Institutes of Health, RePORTER application 3126937, TRANSCRIPTION AND TRANSLATION REGULATION IN INFECTION (5R01AI016954-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3126937. Licensed CC0.

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