# ACYCLOVIR AND OTHER DRUG INHIBITION OF EBV REPLICATION

> **NIH NIH R01** · UNIV OF NORTH CAROLINA CHAPEL HILL · 1987 · $151,810

## Abstract

On the basis of the work done in the first grant period with acyclovir
(ACV) and Epstein-Barr Virus (EBV) we have constructed a project extending
this work and consisting of two parts:  the first describes experiments
designed to evaluate and characterize virologically and biologically the
effects of four promising new antiviral agents (B10LF-62, BVDU, FIAC and
FMAU), all nucleoside analogs and one of them a congener of ACV, on EBV
replication both in isolated nuclei and in cell culture systems.  The
second part deals with the mode of action of the drugs.  In this part based
on what we have learned from our studies with ACV we will investigate drug
metabolism and purify and characterize EBV-specified DNA polymerase, a key
enzyme in the drug interactions, with respect to (i) kinetics of inhibition
by phosphorylated drug, (ii) the ability of drug-triphosphates to serve as
substrate, (iii) effect of incubation on primer-template efficiency, (iv)
question of chain termination, (v) identification of incorporated drug
moieties in EBV DNA, and (vi) reversibility of drug effects correlated with
impact on latent infection.  Data obtained from these studies will
facilitate our understanding of the molecular mechanisms of action of the
drugs and help in the development of selective antiviral agents.

## Key facts

- **NIH application ID:** 3127025
- **Project number:** 5R01AI017205-08
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **SAM.gov UEI:** D3LHU66KBLD5
- **Principal Investigator:** JOSEPH S PAGANO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $151,810
- **Award type:** 5
- **Project period:** 1980-08-01 → 1988-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127025

## Citation

> US National Institutes of Health, RePORTER application 3127025, ACYCLOVIR AND OTHER DRUG INHIBITION OF EBV REPLICATION (5R01AI017205-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127025. Licensed CC0.

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