# CD4 & CD8 IN INDUCTION & SPECIFICITY OF T LYMPHOCYTES

> **NIH NIH R01** · DANA-FARBER CANCER INST · 1988 · $260,658

## Abstract

The general aim of this proposal is to characterize the biological
role played by CD4 and CD8 in T cell activation and to further
define the biological effects of HIV binding to CD4.  The
approaches planned rely on recent methodological developments
in cell biology, protein chemistry, and molecular biology.  In order
to accomplish these aims we have developed a model system in
which cDNAs encoding human T cell surface molecules can be
readily expressed in murine T cell hybridomas that are
specifically stimulated by human class I or Class II MHC antigens.
This system enables us to analyse the functional effects of various
mutations of the cDNAs encoding CD4 and CD8 expressed in an
antigne reactive T cell.  Detailed structure/function studies can
then be performed.

The specific aims of the proposal are as follows:  (1) Determine
whether the ligands for CD4 and CD8 are the class II and class I
MHC antigens, respectively; (2) Determine the role of CD4 and
CD8 in cell adhesion; (3) Determine the role of CD4 and CD8 in
antigen-activated signal transduction; (4) Determine the role of
MAbs directed against CD4 and CD8 in signal transduction; (5)
Determine the role of anti-CD4 and anti-CD8 MAb in negative
signaling; (6) Use CD4 and CD8 mutants to define the relationship
between structure and function; (7) Determine the effects ofthe
binding of HIV gp120 to CD4.

## Key facts

- **NIH application ID:** 3127065
- **Project number:** 2R01AI017258-09
- **Recipient organization:** DANA-FARBER CANCER INST
- **SAM.gov UEI:** DPMGH9MG1X67
- **Principal Investigator:** Steven J. Burakoff
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $260,658
- **Award type:** 2
- **Project period:** 1980-09-01 → 1993-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127065

## Citation

> US National Institutes of Health, RePORTER application 3127065, CD4 & CD8 IN INDUCTION & SPECIFICITY OF T LYMPHOCYTES (2R01AI017258-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127065. Licensed CC0.

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