# INDUCTION AND SPECIFICITY OF CYTOLYTIC T LYMPHOCYTE

> **NIH NIH R01** · DANA-FARBER CANCER INST · 1987 · $187,905

## Abstract

The general aim of my research is to develop the capacity to regulate or
manipulate the cytolytic T lymphocyte (CTL) response.  This interest
extends to understanding the CTL response to viral infections, malignancies
and also to alloantigens (because of the evidence for the involvement of
CTLs in renal and bone marrow transplantation).  In order to accomplish my
more general goal I feel that is necessary to define on a molecular level,
the antigenic requirements for triggering pre-CTLs and the antigens
recognized by CTLs.  Recent methodological advances have made it possible
(1) to clone major histocompatibility complex (MHC) genes; (2) to isolate
and purify MHC and viral antigens that retain CTL stimulating activity when
inserted into artificial membranes or liposomes and (3) to clone helper T
cells and CTLs.  These monoclonal T cell lines provide a powerful probe to
define the T cell recognition site(s) on MHC and viral antigens.
Therefore, the specific aims of my research are as follows:  (1) To utilize
cloned MHC genes to define the antigenic sites on H-2 molecules recognized
by allospecific and virus specific CTLs.  Since there are likely to be
multiple CTL recognition sites on these molecules, cloned allospecific and
virus specific CTLs will be generated.  (2) Cloned viral genes will be used
to define the antigenic sites on viral proteins recognized by virus
specific CTLs.  Specifically, a cloned Mooney murine leukemia virus gp70
gene and a cloned influenza virus hemagglutinin (HA) gene will be
manipulated to assess serological and CTL recognition sites.  (3) Influenza
viral proteins and H-2 proteins will be isolated and introduced into
liposomes to study the antigenic requirements for triggering influenza
virus specific CTLs.  (4) Isolated H-2K/D and Ia antigens will be isolated
and introduced into liposomes to define the antigenic requirements for
triggering cloned T helper and T proliferating cells.

## Key facts

- **NIH application ID:** 3127069
- **Project number:** 5R01AI017258-08
- **Recipient organization:** DANA-FARBER CANCER INST
- **SAM.gov UEI:** DPMGH9MG1X67
- **Principal Investigator:** Steven J. Burakoff
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $187,905
- **Award type:** 5
- **Project period:** 1980-09-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127069

## Citation

> US National Institutes of Health, RePORTER application 3127069, INDUCTION AND SPECIFICITY OF CYTOLYTIC T LYMPHOCYTE (5R01AI017258-08). Retrieved via MORVS 2026-10-26 from https://api.morvs.ai/grant/nih/3127069. Licensed CC0.

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