# CELLULAR GENE EXPRESSION IN ADENOVIRUS-INFECTED CELLS

> **NIH NIH R01** · PRINCETON UNIVERSITY · 1987 · $101,344

## Abstract

The experiments described in this proposal are directed towards elucidation
of the molecular mechanisms whereby adenovirus-infection disrupts cellular
RNA metabolism.  A detailed examination of newly-synthesized, cellular RNA
sequences complementary to human tubulin and histone genes will be
performed by saturation hybridization and size fractionation of both
labelled and unlabelled RNA species.  These experiments are designed to
confirm our previous conclusion that transcription and enzymatic processing
of cellular mRNA precursors is not inhibited in infected cells, even though
newly synthesized, non-viral mRNA sequences fail to enter the cytoplasm.
The packaging of these cellular sequences in ribonucleoprotein structures
will also be examined during the late phase of infection.  To establish
whether discrimination between viral and cellular mRNA sequences occurs at
this step of mRNA biogenesis.  The role of potential trans- and cis-acting
viral functions that might mediate selective appearance in the cytoplasm of
adenoviral mRNA species will be investigated:  certain conditional-lethal
mutants with lesions in early regions E1A and E1B will be screened for
failure to disrupt cellular metabolism and recombinants in which a
selectable gene is placed under control of the adenovirus major late
promoter site will be constructed for introduction into human cells,
respectively.  The unique properties of adenovirus-infected cells will also
be exploited to provide a definitive test for transport of mRNA species
from nucleic isolated by different procedures.

## Key facts

- **NIH application ID:** 3127079
- **Project number:** 5R01AI017265-08
- **Recipient organization:** PRINCETON UNIVERSITY
- **SAM.gov UEI:** NJ1YPQXQG7U5
- **Principal Investigator:** Sarah Jane FLINT
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $101,344
- **Award type:** 5
- **Project period:** 1980-08-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127079

## Citation

> US National Institutes of Health, RePORTER application 3127079, CELLULAR GENE EXPRESSION IN ADENOVIRUS-INFECTED CELLS (5R01AI017265-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127079. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*