# IMMUNOREGULATION IN DISSEMINATED HISTOPLASMOSIS

> **NIH NIH R01** · UNIVERSITY OF CINCINNATI · 1990 · $180,653

## Abstract

Longterm objectives are to study the perturbations of
immunoregulation that are associated with infection by the
pathogenic yeast, Histoplasma capsulatum (Hc).  Studies of
experimental pulmonary infection in mice will focus on the flux of
lymphocyte subpopulations within infected lungs at serial
intervals after intranasal inoculation by means of flow
microfluorometry and immunohistological techniques.  Antigen-
specific vs non-specific recruitment of lymphocytes to infected
lungs will be studied.  These findings will be correlated with other
experiments to assay production of Interleukin I and Interleukin II
by cells from infected lung during the course of infection.  The
role played by T-L3T4+ helper/inducer cells in host defense
against Hc infection and in modulation of the granulomatous
inflammatory response will be examined by administration of the
anti L3T4 monoclonal antibody GK 1.5.

Studies on the interactions between unopsonized Hc yeasts and
human monocyte-derived macrophages will be carried out to
define further the importance of a glycorprotein surface receptor
on M0 as the major site for binding of Hc to macrophages.  More
particularly, the role of the common B chain of the family of
adherence glycoproteins CR3/LFA-1/P150-,95 will be determined.
Factors governing phagocytosis and killing of Hc yeasts will be
analyzed with emphasis on the regulatory effects of Interferon y,
fibronectin, and phorbal myristate acetate.

Ultrastructural studies will be performed by electron microscopy
to examine for the first time, the fate of Hc within human
macrophages.  Mechanisms by which the organism appears to
evade killing will be explored with emphasis on intra-phagosomic
events.

In humans, possible quantitative abnormalities of
immunoregulatory cell populations of peripheral blood will be
examined by flow microfluorometry.  Functional properties of
regulatory cell subsets in peripheral blood will be studied by in
vitro techniques with respect to production of the cytokines IL-1
and IL-2 and INFy in response to specific and nonspecific stimuli.
Similar studies will be performed on cells by bronchoalveolar
lavage of patients with pulmonary histoplasmosis.

## Key facts

- **NIH application ID:** 3127167
- **Project number:** 5R01AI017339-10
- **Recipient organization:** UNIVERSITY OF CINCINNATI
- **SAM.gov UEI:** DZ4YCZ3QSPR5
- **Principal Investigator:** Ward E Bullock
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $180,653
- **Award type:** 5
- **Project period:** 1980-09-01 → 1991-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127167

## Citation

> US National Institutes of Health, RePORTER application 3127167, IMMUNOREGULATION IN DISSEMINATED HISTOPLASMOSIS (5R01AI017339-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127167. Licensed CC0.

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