# ISOLATION AND CHARACTERIZATION OF T CELL RECEPTOR GENES

> **NIH NIH R01** · CALIFORNIA INSTITUTE OF TECHNOLOGY · 1990 · $115,206

## Abstract

We propose to clone and sequence most of the estimated 60 V Beta gene
segments of the human T-cell receptor.  Representative members of the V
Alpha gene segment subfamily will also be cloned and sequenced.  We plan to
map the chromosomal location of these V gene segments using pulsse gradient
gel electrophoresis techniques.  We will construct nucleic acid probes
specific for each of these V gene segments that should allow us to divide
all human T cells into one of 60 categories (corresponding to the 60 V Beta
segments).  We will also attempt to raise specific antibodies against some
of these V segments.  We will use these reagents to identify the V Beta
(and V Alpha) gene segments employed in human T-cell malignancies and in
autoimmune diseases presumably mediated by T cells such as rheumatoid
arthritis and multiple sclerosis.  We are interested in whether these
diseases are mono- or pauciclonal and, if so, in the potential diagnostic
and therapeutic potential of the antibody reagents.  We also plan to
chemically synthesize the T4 homology unit and determine whether it binds
to class II MHC molecules.

## Key facts

- **NIH application ID:** 3127279
- **Project number:** 5R01AI017565-10
- **Recipient organization:** CALIFORNIA INSTITUTE OF TECHNOLOGY
- **SAM.gov UEI:** U2JMKHNS5TG4
- **Principal Investigator:** LEROY E HOOD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $115,206
- **Award type:** 5
- **Project period:** 1981-01-01 → 1991-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127279

## Citation

> US National Institutes of Health, RePORTER application 3127279, ISOLATION AND CHARACTERIZATION OF T CELL RECEPTOR GENES (5R01AI017565-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127279. Licensed CC0.

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