# GENETIC AND BIOCHEMICAL ANALYSIS OF AD 5 REGION 2

> **NIH NIH R01** · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · 1987 · $96,844

## Abstract

The objective is to gain an understanding of the control mechanisms
operative in the regulation of eukaryotic gene expression, using adenovirus
type 5 (Ad5) early region 2A as the model system.  The gene product of
early region 2A is a 72,000 dalton single-stranded DNA-binding protein
(DBP) that is involved in regulating viral gene expression in at least four
ways:  (1) the initiation and elongation steps of DNA synthesis, (2) the
regulation of transcription of several of the Ad5 early genes, (3)
stability of early mRNAs, and (4) the host-range of the virus.

The immediate goal is to precisely define within the DBP domains that are
involved in each of the functions of the protein.  To accomplish this, we
will generate mutations in the DBP by site-specific mutagenesis techniques
and analyze the mutant DBPs for their ability to bind to single-stranded
DNA, and to help adeno-associated virus replicate in human cells.

In-depth probing of the functional properties of the protein will be
performed following re-introduction of the mutated gene back into the virus.

## Key facts

- **NIH application ID:** 3127344
- **Project number:** 5R01AI017654-06
- **Recipient organization:** ST. JUDE CHILDREN'S RESEARCH HOSPITAL
- **SAM.gov UEI:** JL4JHE9SDRR3
- **Principal Investigator:** GEOFFREY R KITCHINGMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $96,844
- **Award type:** 5
- **Project period:** 1980-12-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127344

## Citation

> US National Institutes of Health, RePORTER application 3127344, GENETIC AND BIOCHEMICAL ANALYSIS OF AD 5 REGION 2 (5R01AI017654-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127344. Licensed CC0.

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