# GENETIC FACTORS IN TRANSPLANT SURVIVALS

> **NIH NIH R01** · UNIVERSITY OF MINNESOTA · 1987 · $212,354

## Abstract

Studies proposed in this application deal with two overall areas.  First,
we shall use cloned T lymphocytes to investigate determinants associated
with HLA class II molecules.  Despite the accumulating information at the
genetic and protein levels regarding HLA class II genes and their products,
very little is known about polymorphism, as recognized by T lymphocytes,
associated with the individual alphabeta products.  The polymorphism
defined with homozygous typing cells (HTCs) does not allow conclusions
regarding polymorphism of individual products that carry determinants
contributing to the specificities defined with HTCs.  Products to be
evaluated include HLA-DR alphabeta-1, DR alphabeta-2, and DQ alphabeta.  To
the extent that additional DR or DQ dimers are expressed, or that
additional class II genes exist that are in linkage disequilibrium with
DR/DQ and that are expressed, polymorphism associated with those products
will also be studied.  Both cloned proliferating, non-cytotoxic and
cytotoxic clones will be used.  Our emphasis will be on DR2+ and the DR4+
haplotypes, given the existence of the multiple Dw subtypes of each of
these serologically-defined specificities and the importance of these
haplotypes in a number of associations of HLA and disease.  These studies
will be done in normal individuals and should contribute to the
understanding of HLA as it relates to transplantation.  Second, we plan to
study associations of HLA class II genes/products and insulin-dependent
diabetes (IDD).  Sequencing of expressed class II DR and DQ genes from
patients with IDD will be performed in an attempt to identify sequences
within one or more of those genes that are present more (or less)
frequently in IDD patients than in normal individuals.  Such sequences may
serve as better markers for IDD than are presently available and, to the
extent that these sequences are of import functionally in IDD patients, may
help us to understand the pathogenesis of the disease.  In addition to DNA
sequencing, other methods will be used in an attempt to pinpoint the degree
of conservation/polymorphism of sequences of interest in IDD patients
expressing a given combination of serologically-defined specificities of DR
and DQ.  We consider it a strength of this application that the same cells
proposed herein for study have been and are continuing to be studied by a
variety of methods.

## Key facts

- **NIH application ID:** 3127387
- **Project number:** 5R01AI017687-08
- **Recipient organization:** UNIVERSITY OF MINNESOTA
- **SAM.gov UEI:** KABJZBBJ4B54
- **Principal Investigator:** FRITZ H BACH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $212,354
- **Award type:** 5
- **Project period:** 1980-05-01 → 1991-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127387

## Citation

> US National Institutes of Health, RePORTER application 3127387, GENETIC FACTORS IN TRANSPLANT SURVIVALS (5R01AI017687-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127387. Licensed CC0.

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