# PLASMID REPLICATION AND RECOMBINATION IN E COLI

> **NIH NIH R01** · EMORY UNIVERSITY · 1988 · $219,650

## Abstract

We will continue our studies on control of plasmid DNA replication in E.
coli using P1, a bacteriophage whose prophage is a plasmid, as a model
system.  From P1 carrying multiple antibiotic resistance determinants, we
have isolated mutants that are maintained at multiple copies per cell.  We
plan to analyze these in detail to determine the molecular mechanisms
involved in copy number regulation.  We will also isolate mutants that
overproduce a factor we believe to be required for P1 plasmid replication
and characterize the product they define.  We will study the interaction of
the different regulatory products by suppressor analysis.  We plan to
identify and characterize the RNAs and proteins involved in the regulation
of replication of this model plasmid and to begin work on an in vitro
replication system.

In addition, we will complete our work on the generation of a free
resistance determinant from P1ApCm.

## Key facts

- **NIH application ID:** 3127394
- **Project number:** 5R01AI017696-08
- **Recipient organization:** EMORY UNIVERSITY
- **SAM.gov UEI:** S352L5PJLMP8
- **Principal Investigator:** June R Scott
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $219,650
- **Award type:** 5
- **Project period:** 1981-04-01 → 1990-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127394

## Citation

> US National Institutes of Health, RePORTER application 3127394, PLASMID REPLICATION AND RECOMBINATION IN E COLI (5R01AI017696-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127394. Licensed CC0.

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