# CHAIN GENE ORGANIZATION IN TRANSFORMED B LYMPHOCYTES

> **NIH NIH R01** · UT SOUTHWESTERN MEDICAL CENTER · 1989 · $164,165

## Abstract

The goal of this grant and its predecessor is to study the
molecular events related to heave chain gene expression.  The last
grant focused on the structure and rearrangement of C/H genes.
An in depth characterization of the Mu-C delta locus in mouse
and man has provided a framework to address fundamental issues
of transcriptional and post-transcriptional regulation.  In this
renewal, we propose to extend the work on Mu-delta regulation,
primarily be exploiting in vitro expression technology, with an eye
toward understanding the differential roles of the gene products.
Specifically, we will determine the levels at which IgM and IgD
are regulated in human B cells; significance draws on the
dramatic evolutionary divergence of C delta structure in this
species while retaining phenotypic conservation in IgD function.
We propose a series of transfection studies aimed at fine
dissection of 3' and regulation in the murine mu-delta locus.  That
is, what is the mechanism(s) governing membrane vs.  secreted
carboxyl-terminus and/or Mu vs delta exon selection?  We propose
studies on V/H promoter-associated sequences, focusing on
protein:  DNA interactions and the regulatory effects of T cells
on this interaction.  We propose a novel cDNA cloning strategy
for characterizing putative transcription termination and
promoter-associated factors.  We provide preliminary feasibility
data and design projects for exploitation of antigen-specific IgM
and IgD transfected B cell lines.  Specifically, we will investigate
the role of phosphorylcholine-specific mIg receptors, both in wild-
type and structurally modified forms, in B cell activation,
processing, and antigen presentation.  Finally, we devise a
molecular approach to address the differential roles of mIgM and
IgD in neonatal B cell tolerance.

## Key facts

- **NIH application ID:** 3127636
- **Project number:** 5R01AI018016-10
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **SAM.gov UEI:** YZJ6DKPM4W63
- **Principal Investigator:** HALEY O TUCKER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $164,165
- **Award type:** 5
- **Project period:** 1980-09-01 → 1992-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127636

## Citation

> US National Institutes of Health, RePORTER application 3127636, CHAIN GENE ORGANIZATION IN TRANSFORMED B LYMPHOCYTES (5R01AI018016-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127636. Licensed CC0.

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