# STRUCTURE AND FUNCTION OF ADENOVIRUS GENOME

> **NIH NIH R01** · NORTHWESTERN UNIVERSITY · 1987 · $163,156

## Abstract

The long range goal of this research program is to study the regulation of
gene expression of DNA tumor virus genomes and to use this knowledge to
understand the mechanism of eukaryotic gene expression and virus-host cell
interactions.  During the tenure of the proposed grant the following three
aspects of human adenovirus type 5 gene expression will be studied.

(l) Structure-function relationship of two virus-associated (VA) RNA genes
will be studied to seek the molecular basis of their translation
enhancement of viral mRNAs.  Genetic approaches will be used to examine
whether or not there is a functional correlation between the short
complementary nucleotide sequences present in the VAI RNAs and the
tripartite leader segments of adenoviral late mRNAs.  In vivo nucleic acid
cross linking approaches will be used to see if the VA RNAs interact with
late viral mRNAs during translation.  An adenovirus mutant will be
constructed in which both VAI and VAII RNA genes are deleted.  The
phenotypic analysis of this mutant should reveal if the VA RNAs have
functions in addition to their translation enhancement role.

(2) The biological function of adenoviral early gene (Ll-early gene)
located between 30.0 and 39.0 map units will be examined by constructing
mutant viruses with mutations in the coding region of this gene.

(3) The function of a protein coding sequence present in the i leader of
the major late transcriptional unit will be examined by constructing an
adenovirus point mutant.  This point mutant will prevent translation of the
i leader.

## Key facts

- **NIH application ID:** 3127644
- **Project number:** 5R01AI018029-07
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **SAM.gov UEI:** KG76WYENL5K1
- **Principal Investigator:** BAYAR THIMMAPAYA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $163,156
- **Award type:** 5
- **Project period:** 1981-07-01 → 1988-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127644

## Citation

> US National Institutes of Health, RePORTER application 3127644, STRUCTURE AND FUNCTION OF ADENOVIRUS GENOME (5R01AI018029-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127644. Licensed CC0.

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