# REGULATION & SPECIFICITY OF THE IMMUNE RESPONSE TO VSV-G

> **NIH NIH R01** · NEW YORK UNIVERSITY · 1991 · $152,292

## Abstract

Using the model system of the murine immune response to the vesicular
stomatitis virus (VSV) G protein, studies on the regulation and specificity
of the immune response to viruses will be performed.  These studies will
provide critical insights into cellular immunology.

Fine structural analysis of the thymus derived (T cell ) lymphocyte clones
and hybridomas elicited in immunized mice and the epitopes they recognize
will be undertaken.  Both CD4+ and CD8+ G-reactive T cells will be studied;
the MHC restriction of the epitopes recognition will be defined.  The
sequences recognized by the T cells will be characterized by a variety of
methods including:  infections vs. killed virus wild type vs. defective
interfering virus, purified G protein micelles or isolated trimers of
truncated soluble Gs, serotype (NJ vs Indiana) restriction, field isolates,
vaccinia-G vectors, transfected antigen presenting cells (APCs),
proteolytic peptides of G, lacZ fusion proteins, synthetic oligopeptides.
Cytokines secreted upon stimulation will be characterized by bioassay,
ELISA, or in situ hybridization.

The clones/hybridomas will be characterized for utilization of T cell
receptor (TCR) variable region genes, and anti-idiotypic (anti-ID) reagents
will be generated and used in studies of immunization and perturbation of
the hybridomas will be studied both in vitro and in vivo.  The influence of
the hosts genotype on the epitopes recognized and the IDs utilized will be
examined.  Whether T cell or B cell immunity, or a combination of both is
critical for immune protection will be studied.  The influence of loss of
principal restricting elements on the hosts' response will be investigated.

The techniques used in the pursuit range from basic cellular immunologic
culture and assays of T cells, to molecular cloning and transfections, in
situ hybridization, biochemical isolation of fragments and fusion proteins,
cell biology viral infection of living hosts, and viral propagation in
vitro.

## Key facts

- **NIH application ID:** 3127674
- **Project number:** 7R01AI018083-11
- **Recipient organization:** NEW YORK UNIVERSITY
- **SAM.gov UEI:** NX9PXMKW5KW8
- **Principal Investigator:** CAROL SHOSHKES REISS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $152,292
- **Award type:** 7
- **Project period:** 1981-08-01 → 1995-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127674

## Citation

> US National Institutes of Health, RePORTER application 3127674, REGULATION & SPECIFICITY OF THE IMMUNE RESPONSE TO VSV-G (7R01AI018083-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127674. Licensed CC0.

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