# VACCINIA VIRUS BIOCHEMICAL GENETICS

> **NIH NIH R01** · UNIVERSITY OF FLORIDA · 1993 · $225,921

## Abstract

The poxvirus vaccinia, which is the live vaccine virus used in the
eradication of smallpox, is a large double stranded DNA containing virus
that replicates in the has been to understand genome using a genetic
vaccinia has uncovered be critical for proper includes vaccinia gene
increased steady state cytoplasm of infected cells.  Our long-range goal
the functional organization of the vaccinia approach.  Our ongoing
genetic analysis of a network of essential viral genes which seem to
control of late viral transcription.  The network A18R, which when
mutated leads to synthesis of levels of late viral RNA, and gene G2R,
which when mutated leads to decreased synthesis of late viral proteins.
In this application, we propose to extend the analysis of this network
and continue our efforts to develop directed in vitro methods for
construction of conditionally lethal mutations in essential vaccinia
genes.  The specific aims are: 1) optimize and characterize a virus
specific in vitro transcription system, 2) using biochemical protocols
and appropriate mutants, characterize the product of the A18R gene and
determine its role in infection, 3) using biochemical protocols and
appropriate mutants, characterize the product of the G2R gene and
determine its role in infection, 4) using genetic protocols, determine
.the relationship between genes A18R and G2R, and seek additional genes
involved in the network, and 5) test alanine scanning mutagenesis coupled
with transient dominant selection as a method for directed in vitro
construction of conditionally lethal mutants of vaccinia.

## Key facts

- **NIH application ID:** 3127677
- **Project number:** 2R01AI018094-12A1
- **Recipient organization:** UNIVERSITY OF FLORIDA
- **SAM.gov UEI:** NNFQH1JAPEP3
- **Principal Investigator:** Richard C Condit
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $225,921
- **Award type:** 2
- **Project period:** 1990-07-01 → 1998-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127677

## Citation

> US National Institutes of Health, RePORTER application 3127677, VACCINIA VIRUS BIOCHEMICAL GENETICS (2R01AI018094-12A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127677. Licensed CC0.

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