# VACCINIA VIRUS BIOCHEMICAL GENETICS

> **NIH NIH R01** · UNIVERSITY OF FLORIDA · 1991 · $201,513

## Abstract

The long term goals of this project are to understand the
functional organization of the vaccinia virus genome, the
mechanisms by which vaccinia virus gene expression is regulated,
and the interactions between vaccinia virus and the host cell.  The
approach taken to achieve these goals is the isolation and
functional analysis of conditionally lethal mutants of the virus.
Specifically, this application proposes to characterize in detail
previously isolated temperature sensitive mutations which seem
to affect three distinctly different aspects of vaccinia biology.
Viral transcription will be addressed by studying several
temperature sensitive mutations affecting two of the viral RNA
polymerase subunits.  Post transcriptional regulation of RNA
stability will be addressed by studying temperature sensitive, drug
resistant, and drug dependent mutants all related to an "abortive
late" mutant phenotype.  Virion morphogenesis will be studied by
analyzing a large group of temperature sensitive mutants which
confer a "normal" phenotype on an infection done at the non-
permissive temperature.

This application also proposes to continue the development of
methods for site directed in vitro mutagenesis of vaccinia genes.
Using a recently developed protocol for efficient insertion of
unselected markers into the vaccinia genome, attempts will be
made to construct temperature sensitive mutations in specific
viral genes after chemical mutagenesis of defined viral DNA's in
vitro.  Experiments will also be performed to test the usefulness
of a newly constructed nonsense suppressing cell line for the
isolation of nonsense mutants of vaccinia virus.

## Key facts

- **NIH application ID:** 3127682
- **Project number:** 5R01AI018094-11
- **Recipient organization:** UNIVERSITY OF FLORIDA
- **SAM.gov UEI:** NNFQH1JAPEP3
- **Principal Investigator:** Richard C Condit
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $201,513
- **Award type:** 5
- **Project period:** 1990-07-01 → 1993-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127682

## Citation

> US National Institutes of Health, RePORTER application 3127682, VACCINIA VIRUS BIOCHEMICAL GENETICS (5R01AI018094-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127682. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*