# STUDIES OF GLYCOPROTEIN D OF HERPES SIMPLEX VIRUS

> **NIH NIH R01** · UNIVERSITY OF PENNSYLVANIA · 1988 · $175,774

## Abstract

The objective of the proposed investigation is to carry out a detailed
biochemical and immunological study of the type-common envelope
glycoprotein D of herpes simplex virus (HSV) type l (oral form) and HSV-2
(genital form).  Many structural and immunological properties of gD
indicate that it is an important candidate for consideration as a subunit
vaccine.  We plan to localize, construct and characterize antigenic
determinants of gD.  We will characterize monoclonal antibodies from a
number of laboratories in an effort to identify additional gD epitopes.
Antibodies will be tested against a variety of fragments derived from gD,
to facilitate mapping.  In addition, synthetic peptides corresponding to
the probable locations of specific epitopes will be synthesized and tested
for antigenicity production of neutralizing antibody, and ability to
stimulate a protective immune response in mice.  Monoclonal antibody
resistant (mar) mutants will be analyzed in an effort to further localize
gD epitopes.  Experiments are proposed to determine the number and location
of disulfide bridges in gD-l and gD-2.  These studies will be of importance
in understanding aspects of the secondary and tertiary structure of gD.
These structural studies will enable us to understand the nature of
conformational epitopes.  Preparations of gD-l and gD-2 and fragments
derived from these proteins will be evaluated for their protective capacity
in a mouse virus challenge model system.  We will assess the relationship
between stimulation of neutralizing antibody and protection against lethal
virus challenge.  The studies proposed in this grant will permit an
assessment to be made of the usefulness of glycoprotein D or a derivative
of it in stimulating a protective immune response.

## Key facts

- **NIH application ID:** 3127815
- **Project number:** 5R01AI018289-08
- **Recipient organization:** UNIVERSITY OF PENNSYLVANIA
- **SAM.gov UEI:** GM1XX56LEP58
- **Principal Investigator:** GARY H COHEN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $175,774
- **Award type:** 5
- **Project period:** 1981-09-30 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127815

## Citation

> US National Institutes of Health, RePORTER application 3127815, STUDIES OF GLYCOPROTEIN D OF HERPES SIMPLEX VIRUS (5R01AI018289-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127815. Licensed CC0.

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