# IDIOTYPY OF ANTI-INFLUENZA VIRUS IMMUNE RESPONSE

> **NIH NIH R01** · MOUNT SINAI SCHOOL OF MEDICINE OF CUNY · 1987 · $213,756

## Abstract

The overall goal of this proposal is to investigate the origin of diversity
of murine anti-PR8 influenza virus immune responses and to evaluate the
effects of anti-Id antibodies in regulating these responses and in
particular host defense mechanisms, in general.  We have already obtained
and identified monoclonal antibodies (MAb) specific for various epitopes of
hemagglutinin (HA) and the HA2 subunit of PR8 virus, and by
immunoprecipitation have characterized MAbs specific for viral
neuraminidase (NA) and nucleoprotein (NP).  In addition, we are in the
process of preparing MAbs against laboratory selected antigenic variants
and MAbs specific for viral HA expressed in infected cells.  We now propose
to extend our observations on the expression of cross-reactive idiotypes
(IdX) to these antibodies.  In addition,, helper and cytolytic T cell
clones specific for viral HA are being prepared.  The presence of IdX among
MAbs specific for HA and other viral antigens and on the antigen receptor
of T cell clones would indicate a common germ line gene from which
antibodies and T cell receptors are derived.  In further studies, we
propose to analyze by Northern blotting under conditions of low and high
stringency the hybridization of various germ line gene probes with mRNA
from hybridomas secreting MAbs specific for different viral antigens which
do or do not express IdXs, and then to sequence the RNA from the hybridomas
using VK21 and different VII germ line genes.

Furthermore, we will prepare monoclonal anti-Id antibodies to study their
ability to alter the immune response against PR8.  In these studies, the
effects of monoclonal anti-Id antibodies on the immune response in vitro
and on the defense reactions of BALB/c mice infected with PR8 influenza
virus will be studied.

Finally, we will also determine which of these anti-Id MAbs carry the
internal image of the HA and thus are able to prime the animal or to evoke
a secondary response in a primed animal.

## Key facts

- **NIH application ID:** 3127841
- **Project number:** 5R01AI018316-07
- **Recipient organization:** MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
- **Principal Investigator:** CONSTANTIN A BONA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $213,756
- **Award type:** 5
- **Project period:** 1981-08-01 → 1989-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127841

## Citation

> US National Institutes of Health, RePORTER application 3127841, IDIOTYPY OF ANTI-INFLUENZA VIRUS IMMUNE RESPONSE (5R01AI018316-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127841. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*