# CHEMISTRY OF PHAGE INVASION, STRUCTURE, AND ASSEMBLY

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 1989 · $157,441

## Abstract

The T-even bacteriophage - E. coli system will be investigated as a model
system of viral invasion, viral structure and viral assembly.  Recent
results have been identified a number of phage genes which produce a set of
enzymes which ultimately form a unique type of folic acid - a
dihydropteroyl hexaglutamate.  In addition these enzymes have been found to
be structural components of the central hub of the tail baseplate of the
phage particle.

Work will be devoted to determine:

1) The pathway or sequence of the phage gene products take to assemble the
central baseplate hub;

2) The nature of the interaction of these hub components with each other
during the formation of phage-induced folate;

3) Development of new methods to determine the assembly sequence pathway
including the use of inhibitors to accumulate intermediates and selective
labeling of different hub components.

4) The nature of the interaction of the hub components with host cell
membrane.

5) The nature of the interaction of these hub components with the tail
fibers especially the interaction of T4 gene product 28 with the
zinc-containing short tail fiber.

It is hoped that the detailed chemical knowledge gained from these studies
will lead to the control of virus assembly and to new principles involving
assembly of other viruses including those causing human diseases.

## Key facts

- **NIH application ID:** 3127867
- **Project number:** 5R01AI018370-09
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **SAM.gov UEI:** KMH5K9V7S518
- **Principal Investigator:** LLOYD M KOZLOFF
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $157,441
- **Award type:** 5
- **Project period:** 1981-02-01 → 1991-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127867

## Citation

> US National Institutes of Health, RePORTER application 3127867, CHEMISTRY OF PHAGE INVASION, STRUCTURE, AND ASSEMBLY (5R01AI018370-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127867. Licensed CC0.

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