# LIPOPOLYSACCHARIDES OF N GONORRHOEAE

> **NIH NIH R01** · STATE UNIVERSITY OF NEW YORK AT BUFFALO · 1987 · $144,510

## Abstract

This proposal represents a continuation of our studies into the
immunobiology of the lipopolysaccharide of Neisseria gonorrhoeae.  These
studies will be accomplished through the following specific aims.
Secretions from men with gonococcal urethritis and secretions and
endocervical tissue from females with gonococcal cervicitis will be studied
to define the histopathology of gonorrhoeae using electron microscopic
methods and to define the antigenic characteristics of lipopolysaccharide
present in human gonococcal infection.  This will be accomplished using
monoclonal antibodies in immunofluorescent, immunoelectron microscopic and
ELISA systems.  2) The chemical nature of the gonococcal LPS antigens which
are present in human disease and which are targets for the human immune
response will be defined.  The oligosaccharide chains containing these
antigens will be isolated and the chemical constituents of these antigens
will be determined.  Oligosaccharide chains shown to contain antigens
relevant to the human immune response will be conjugated to a variety of
gonococcal cell surface proteins including fragments from enzymatic
digestion of pilus and protein l. The human immune response to gonococcal
lipopolysaccharide will be studied to define the LPS sites against which
bactericidal antibody is directed in normal human serum, in patients with
genital gonorrhoeae and in patients with gonococcal PID and disseminated
infection and to define the evolution of the human immune response to
gonococcal LPS which occurs in genital and extra-genital gonococcal
infection.

## Key facts

- **NIH application ID:** 3127893
- **Project number:** 5R01AI018384-07
- **Recipient organization:** STATE UNIVERSITY OF NEW YORK AT BUFFALO
- **SAM.gov UEI:** LMCJKRFW5R81
- **Principal Investigator:** Michael A. Apicella
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $144,510
- **Award type:** 5
- **Project period:** 1981-07-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127893

## Citation

> US National Institutes of Health, RePORTER application 3127893, LIPOPOLYSACCHARIDES OF N GONORRHOEAE (5R01AI018384-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127893. Licensed CC0.

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