# NUCLEIC ACID STUDIES OF ANTI-ARSONATE HYBRIDOMAS

> **NIH NIH R01** · UT SOUTHWESTERN MEDICAL CENTER · 1988 · $133,111

## Abstract

The goals of this research project have been to understand
something of the germline contribution to the anti-arsonate
immune response in A/J mice.  Toward the end, we have cloned
and sequenced the VH, DH, JH and VK and JK germline gene
segments that give rise to this response.  In addition, we have
cloned and sequenced eleven related VH and three related VK
germline gene segments.  A considerable amount of attention was
paid to the organization of the VH locus in the A/J strain and
using deletion mapping, the J558 gene family (to which the Ars-A
VH gene segment belongs) was oriented relative to other gene
families.  In addition, the 3660 VH family (to which the Ars-C VH
gene segment belongs) was also oriented within the A/J VH locus.
We detected several differences between the orientation of the
A/J VH complex and the accepted Balb/c organization.  Further
studies are planned to explore A/J V gene segment organization
and control.

Studies directed toward an understanding of transcription
initiation focused on nuclear proteins that bind to immunoglobulin
VH octamer sequences.  The arsonate as well as the BCL1 systems
were used in this regard and during the next five years, a
considerable effort will be made toward further defining the
proteins involved in this interaction as well as cloning the genes
that encode them.  By this means we hope to understand more of
transcriptional regulation of immunoglobulin genes.

A constant correlation has been and continues to be made
between the germline component of the arsonate idiotypic system
and the expressed proteins.  Experiments are described in which
this relationship is explored from the perspective of N segment
diversity, junctional diversity, gene conversion, and somatic
mutation.

## Key facts

- **NIH application ID:** 3127989
- **Project number:** 5R01AI018499-07
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **SAM.gov UEI:** YZJ6DKPM4W63
- **Principal Investigator:** J Donald Capra
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $133,111
- **Award type:** 5
- **Project period:** 1982-09-30 → 1992-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127989

## Citation

> US National Institutes of Health, RePORTER application 3127989, NUCLEIC ACID STUDIES OF ANTI-ARSONATE HYBRIDOMAS (5R01AI018499-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127989. Licensed CC0.

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