# NUCLEIC ACID STUDIES OF ANTI-ARSONATE HYBRIDOMAS

> **NIH NIH R01** · UT SOUTHWESTERN MEDICAL CENTER · 1993 · $170,044

## Abstract

The original goals of this project concerned the expression of
anti-arsonate hybridomas.  In particular, we hoped to understand
something of the germline contribution to the anti-arsonate immune
response.  These goals have been largely met. During the past five years,
this grant also funded work concerning transcription initiation through
the VH-promoter-associated octamer motif.  The VH genes of the arsonate
system as well as other murine VH and VL genes were used to gain insight
into the factors that regulated immunoglobulin gene expression.

The present application is wholly directed toward this latter issue and
is divided into three aims: First, we seek to continue our studies on the
"conventional" ubiquitous octamer binding protein, OTF1, which promotes
B-cell stage-specific transcription in lieu of OTF2.  Aims 2 and 3 focus
on two types of "unconventional" octamer-binding proteins (i.e., lack the
prototypic POU domain of OTF1, OTF2 and related factors).  One of these,
P3, represents a new class of DNA-binding proteins and shares striking
homology with a Drosophila protein involved in visual processing.  The
other is the well-known Ku antigen, a DNA-associated nuclear protein
recognized by sera from patients with certain autoimmune diseases.

We propose studies of both a molecular biological as well as a
biochemical nature that should provide insight into the mechanisms of
promoter region function in the murine immune and nervous systems.

## Key facts

- **NIH application ID:** 3127993
- **Project number:** 5R01AI018499-12
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **SAM.gov UEI:** YZJ6DKPM4W63
- **Principal Investigator:** HALEY O TUCKER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $170,044
- **Award type:** 5
- **Project period:** 1982-09-30 → 1997-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3127993

## Citation

> US National Institutes of Health, RePORTER application 3127993, NUCLEIC ACID STUDIES OF ANTI-ARSONATE HYBRIDOMAS (5R01AI018499-12). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3127993. Licensed CC0.

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