# HYDROGEN EXCHANGE AND INTERACTIONS OF NUCLEOTIDES

> **NIH NIH R01** · UNIVERSITY OF MISSOURI KANSAS CITY · 1988 · $101,792

## Abstract

In previous work the rate of isotopic hydrogen exchange of the purine ring
was determined for a collection of mono-, oligo-, and polynucleotide
structures using techniques of laser Raman spectroscopy.  The exchange
kinetics were correlated with the type of nucleotide, the molecular
conformation, and the hydrogen bonding interactions of the purine ring.  It
was determined that the exchange rate can be employed to monitor the kind
and amount of DNA or RNA secondary structure, including the number of
strands in multi-stranded helices and the presence of Hoogsteen-type
interactions.  The solvent accessibility of the 7N ring site has emerged as
a particularly influential factor in determining the kinetics of 8C-H
exchange.

In the proposed work the Raman dynamic probe will be exploited to determine
the molecular environment of A and G residues of viral RNA and DNA in
native plant and bacterial viruses, and in partially assembled and mutant
virions.  We shall also investigate several well characterized
nucleoprotein complexes, such as the fd virus gene 5 protein-DNA complex,
the phage lambda (cI and Cro) repressor-operator and E. coli lac
repressor-operator systems.  The kinetic studies will be augmented with
equilibrium Raman studies to determine changes in DNA and RNA structure
which may accompany protein binding.  These investigations will provide new
information on the nature of protein-nucleic acid interactions which are
important for virus assembly and stability, and for gene regulation.  The
project will improve our understanding of the nature of protein/nucleic
acid recognition.

We shall employ the powerful techniques of optical multichannel analysis
and Fourier deconvolution for rapid and accurate data acquisition and
analysis.  The proposed applications will advance the use of laser Raman
spectroscopy both as a complement to other structure-determining methods
and as a quantitative probe of nucleic acid structures and dynamics.

## Key facts

- **NIH application ID:** 3128166
- **Project number:** 5R01AI018758-08
- **Recipient organization:** UNIVERSITY OF MISSOURI KANSAS CITY
- **SAM.gov UEI:** J9CDGR596MN3
- **Principal Investigator:** George J Thomas
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $101,792
- **Award type:** 5
- **Project period:** 1987-09-01 → 1990-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128166

## Citation

> US National Institutes of Health, RePORTER application 3128166, HYDROGEN EXCHANGE AND INTERACTIONS OF NUCLEOTIDES (5R01AI018758-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128166. Licensed CC0.

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