# MOLECULAR BIOLOGY OF THE HEPATITIS B-TYPE VIRUSES

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 1987 · $146,334

## Abstract

We propose to continue our studies of the molecular basis of the
replication and gene expression of the hepatitis B viruses of man and
animals.  Our efforts will continue to center on the ground squirrel
hepatitis virus (GSHV) as a model system in which to pursue the following
objectives:  (1) to develop a genetic system for mutational analysis of
essential viral genes (2) to isolate and characterize the protein products
of these genes (3) to elucidate the structures and coding potential of
viral mRNA's, and to identify the viral transcript which serves as the
putative template for reverse transcription (4) to identify and
characterize transcriptional regulatory signals governing the production of
these RNA's and (5) to develop cell culture systems for the analysis of
viral replication and gene expression.

Our program involves the isolation and characterization of intrahepatic DNA
and RNA using subcellular fractionation, blot-hybridization, S1 nuclease
mapping and molecular cloning techniques.  Viral proteins will be
identified and studied using antisera raised to synthetic peptides and
fusion proteins generated in prokaryotic hosts.  The ability of cloned DNA
to initiate infection of animal hosts will be used to score the phenotype
of mutant or recombinant genomes generated in vitro, as a functional test
of the activity of previously uncharacterized viral genes.

## Key facts

- **NIH application ID:** 3128197
- **Project number:** 5R01AI018782-06
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **SAM.gov UEI:** KMH5K9V7S518
- **Principal Investigator:** DON GANEM
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $146,334
- **Award type:** 5
- **Project period:** 1982-09-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128197

## Citation

> US National Institutes of Health, RePORTER application 3128197, MOLECULAR BIOLOGY OF THE HEPATITIS B-TYPE VIRUSES (5R01AI018782-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128197. Licensed CC0.

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