# CHARACTERISTICS OF T CELL RECEPTORS

> **NIH NIH R01** · NATIONAL JEWISH HEALTH · 1988 · $138,589

## Abstract

This application is designed to study various properties of T cell
receptors.  We will concentrate on those receptors which recognize antigen
in association with major histocompatibility complex (MHC) products
(antigen/H-2) in the mouse.  Our ultimate goals are to identify and isolate
the molecule(s) responsibile for this recognition and to map them
genetically.  We will be greatly helped in these studies by the recent
development in our laboratory of techniques for preparing cloned ?T cell
hybridomas which recognize antigen/H-2 products.  These hybridomas grow
continuously and rapidly in normal tissue culture medium.  When confronted
with the appropriate antigen/H-2 combination, however, they secrete
interleukin-2.  They also bind to antigen-pulsed presenting cells of the
appropriate H-2 haplotype.  Attempts will be made to characterize the
antigen/H-2 receptor(s) immunochemically and biochemically.  Immunochemical
analysis will include studies on antisera and B hybridomas raised against
the T cell hybridomas, with particular emphasis on those which block
antigen binding.  Biochemical analysis will include attempts to isolate
antigen and/or H-2 binding material from radiolabelled cells.  In
collaboration with Dr. Hood we will investigate the expression of Ig
heavy-like mRNA in these cells.  Phosphoryl choline (PC)-reactive
hybridomas will be made to check the status of VH genes and mRNA coding for
PC-binding heavy chains.  In collaborative studies with Dr. Riblet we will
prepare hybrids which can be used for analysis of heavy, kappa and lambda
gene rearrangements.  Somatic cell genetics will be used to establish which
chromosomes code for the T cell receptor(s) for antigen/H-2.  T cell
hybridomas will be prepared using antigen specific parental T cells with
marked chromosomes, and in sublines which retain or have lost antigen
specificity the coincidence of specificity and the marked chromosomes will
be noted.  In addition, in collaboration with Dr. Francke, antigen
non-reactive variants of already existing hybridomas will be analyzed for
chromosome loss.

## Key facts

- **NIH application ID:** 3128205
- **Project number:** 5R01AI018785-07
- **Recipient organization:** NATIONAL JEWISH HEALTH
- **SAM.gov UEI:** VLQKFEEJ3NE9
- **Principal Investigator:** JOHN W KAPPLER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $138,589
- **Award type:** 5
- **Project period:** 1982-05-01 → 1992-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128205

## Citation

> US National Institutes of Health, RePORTER application 3128205, CHARACTERISTICS OF T CELL RECEPTORS (5R01AI018785-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128205. Licensed CC0.

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