# SOMATIC DNA RECOMBINATION IN ANTIBODY GENES

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA BERKELEY · 1987 · $141,097

## Abstract

The purpose of this research proposal is to analyze the molecular
mechanisms of somatic DNA recombination in lymphocytes.  The recombination
occurs in the gene system for the antigen-recognizing molecules of
differentiating lymphocytes.  Specific aims of this proposed project
includes:

1) To further purify an endonuclease which specifically cleaves Ig DNA
segments around the recombination site.

2) To isolate and characterize DNA binding proteins which recognize two
consesus sequences at the recombination site.

3) To investigate a hypothetical RNA molecule which might hold two Ig gene
segments together during recombination.

4) To establish an in vitro recombination system for V-(D)-J joining.

5) To establish a retroviral vector system to analyze the Ig gene
recombination.

6) To examine the Ig heavy-chain and T-cell receptor B-chain genes for the
formation of non-germline elements, which occurs at the joining sites of V,
D and J segments.

In addition to standard molecular cloning and DNA sequencing techniques, we
will make use of the recombinant retroviral vector system for introducing
defined Ig and T-cell receptor gene segments to various cell types of the
lymphocyte lineages.

It is hoped that the proposed research will contribute to our understanding
of lymphocyte differentiation in molecular terms, but also to the
clerification of complex recombination processes required for the
activation of antibody genes.

## Key facts

- **NIH application ID:** 3128213
- **Project number:** 5R01AI018790-06
- **Recipient organization:** UNIVERSITY OF CALIFORNIA BERKELEY
- **SAM.gov UEI:** GS3YEVSS12N6
- **Principal Investigator:** HITOSHI SAKANO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $141,097
- **Award type:** 5
- **Project period:** 1982-08-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128213

## Citation

> US National Institutes of Health, RePORTER application 3128213, SOMATIC DNA RECOMBINATION IN ANTIBODY GENES (5R01AI018790-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128213. Licensed CC0.

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