# POLYSACCHARIDE ANTIGENS OF CRYPTOCOCCUS NEOFORMANS

> **NIH NIH R01** · GEORGIA STATE UNIVERSITY · 1987 · $83,920

## Abstract

An immunochemical approach to the characterization of the capsular
polysaccharides of Cryptococcus neoformans is proposed.  Heteroglycans from
several chains of the four recognized serotypes and mutants partially
blocked in capsule synthesis will be fractionated based on differential
complexation with cetyltrimethylammonium bromide, ion exchange
chromatography, lectin affinity, and immunoaffinity.  The native
polysaccharides, polysaccharide derivatives and oligosaccharides derived by
special chemical treatments, e.g., lithium:  ethylenediamine, anhydrous
hydrofluoric acid and periodate oxidation, will be examined by various
analytical methods, including gas liquid chromatography, high field nuclear
magnetic resonance spectrometry, gas liquid chromatography-mass
spectrometry, and glycosidase susceptibility.  The data obtained will be
used to make linkage and sequence assignments for the native
polysaccharides.

Purified and immunochemically defined capsular polysaccharides and their
derivatives will be used in the development of an enzyme immunoassay
(EIA).  This assay in conjunction with monoclonal antibodies will be used
to delineate the chemical nature of the type specific and other
immunodominant epitopes of C. neoformans.  Structurally defined cryptoccal
polysaccharides, their derivatives and model compounds will be studied by
both direct and hapten inhibition variations of EIA.

## Key facts

- **NIH application ID:** 3128273
- **Project number:** 5R01AI018877-03
- **Recipient organization:** GEORGIA STATE UNIVERSITY
- **SAM.gov UEI:** MNS7B9CVKDN7
- **Principal Investigator:** ROBERT CHERNIAK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $83,920
- **Award type:** 5
- **Project period:** 1985-09-01 → 1989-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128273

## Citation

> US National Institutes of Health, RePORTER application 3128273, POLYSACCHARIDE ANTIGENS OF CRYPTOCOCCUS NEOFORMANS (5R01AI018877-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128273. Licensed CC0.

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