# MOLECULAR BIOLOGY OF MOT AND THE CHE GENE PRODUCTS

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT CHICAGO · 1993 · $216,397

## Abstract

Signal transduction in bacterial chemotaxis is arguably the best understood
of the two-component signalling system first described by Ausubel (Nixon et
al. 1986).  Therefore, the understanding of the underlying mechanisms will
have a great impact in many other examples of response to environmental
signals.

A long term goal of this application is characterization of the physical
interactions and submolecular architecture of the chemotaxis components.
The physical protein-protein interactions are being identified by the
isolation of complexes of the chemotaxis proteins.  These complexes are
being isolated by antibody precipitation and protein affinity
chromatography.  The role of these complexes in the signalling process will
be assessed by examining the composition of these complexes in different
mutant backgrounds and different signalling states.  Changes in biochemical
properties such as phosphorylation will also be examined with respect to
changes in the composition of these complexes.  The elucidation of the
submolecular architecture of these proteins will continue by using a
combination of genetic selection, complex isolation and structural
chemistry to map sites of interaction with other proteins and sites of
function.  This novel combination of molecular genetics and structural
chemistry will produce testable hypotheses of protein structure and
function which could not have been predicted from a purely structural or
genetic approach.

The other long term goal of this application is characterization of the
growth-phase regulated expression of the flagellar regulon cascade.  In
particular, the roles of the positive trans-acting regulators, FlhD and
FlhC, will be studied and their potential involvement in other
postexponentially regulated phenomena will be further explored.

## Key facts

- **NIH application ID:** 3128390
- **Project number:** 5R01AI018985-12
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **SAM.gov UEI:** W8XEAJDKMXH3
- **Principal Investigator:** PHILIP MATSUMURA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $216,397
- **Award type:** 5
- **Project period:** 1982-04-01 → 1995-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128390

## Citation

> US National Institutes of Health, RePORTER application 3128390, MOLECULAR BIOLOGY OF MOT AND THE CHE GENE PRODUCTS (5R01AI018985-12). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128390. Licensed CC0.

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