# MECHANISTIC STUDIES OF GENETIC RECOMBINATION

> **NIH NIH R01** · NORTHWESTERN UNIVERSITY · 1987 · $162,133

## Abstract

The overall goal of this proposed research is to contribute to the
molecular understanding of the mechanism of genetic recombination.  The
approach will involve the investigation of the physical, biochemical, and
enzymatic properties of purified proteins which are known to be involved in
the biological recombination process.  The focus of these studies will be
on the E. coli recA protein, and the interaction with its nucleic acid
substrates, as well as the interaction with other proteins involved in
recombination, such as SSB protein, recBC, and DNA helicases.  The overall
objective of these studies is to understand, at the physical mechanistic
level, how these proteins interact with each other and with their nucleic
acid substrates to produce a recombinant DNA molecule.  The approaches to
be used include physical investigations of the structural equilibrium and
kinetic aspects of binding of recA protein to nucleic acid substrates and
of the mechanism of recA protein catalyzed reactions such as DNA
renaturation and DNA strand assimilation; biochemical studies of mutant
recA protein variants, of proteolytic fragments of recA protein and of
truncated recA gene products; and physical and enzymatic studies of the
interaction of recA protein with other proteins such as SSB protein, recBC,
and DNA helicases.

## Key facts

- **NIH application ID:** 3128394
- **Project number:** 5R01AI018987-06
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **SAM.gov UEI:** KG76WYENL5K1
- **Principal Investigator:** Stephen Charles Kowalczykowski
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $162,133
- **Award type:** 5
- **Project period:** 1982-04-01 → 1990-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128394

## Citation

> US National Institutes of Health, RePORTER application 3128394, MECHANISTIC STUDIES OF GENETIC RECOMBINATION (5R01AI018987-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128394. Licensed CC0.

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