# CORONAVIRUS REPLICATION

> **NIH NIH R01** · U.S.UNIFORMED SERVICES UNIV  HLTH SCIS · 1987 · $81,845

## Abstract

Coronaviruses are a common cause of respiratory infection in man and cause
many diseases in domestic animals, including respiratory and enteric
infections, neurological disease and hepatitis.  Because of the difficulty
of isolating coronaviruses from clinical specimens, the full spectrum of
coronavirus-induced disease in man is not yet known.  We will explore
interactions of a murine coronavirus with different host cells to determine
the molecular basis for its strong species and tissue tropisms.

We will analyze the structural and functional domains of the 3 major
proteins in mouse hepatitis virus, using a large number of monoclonal
antibodies which we have developed as well as antibodies to synthetic
peptides mimicking important regions of viral peptides.  We will determine
the roles in virus replication of virus-specific RNA-binding proteins and a
newly discovered 140K virion-associated protein.

We will explore the assembly of these enveloped virions at intracytoplasmic
membranes by biochemical, immunological and electron microscopic
techniques.  We will make use of virus mutants defective in assembly,
infection of hybridoma cells producing antibodies to viral proteins, and
microinjection of monoclonal antibodies into living cells.

Using a variety of susceptible and resistant cell types, we will analyze
the cellular mechanisms for restriction of coronavirus replication.  We
will compare virus attachment and penetration in these different cell
types, and use a new in vitro assay to learn whether differences in
transcription of viral RNA and translation of viral proteins can account
for differential susceptibility of cells to coronavirus infection.  We will
determine the role in coronavirus maturation of host cell lipids and
host-dependent proteolytic cleavage of a viral glycoprotein.

## Key facts

- **NIH application ID:** 3128414
- **Project number:** 5R01AI018997-06
- **Recipient organization:** U.S.UNIFORMED SERVICES UNIV  HLTH SCIS
- **SAM.gov UEI:** Z83LJK5NWBC1
- **Principal Investigator:** Kathryn V Holmes
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $81,845
- **Award type:** 5
- **Project period:** 1982-04-01 → 1990-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128414

## Citation

> US National Institutes of Health, RePORTER application 3128414, CORONAVIRUS REPLICATION (5R01AI018997-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128414. Licensed CC0.

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