# MOLECULAR STUDIES OF NK CELLS AND NK/LAK FUNCTION

> **NIH NIH R01** · UNIVERSITY OF MINNESOTA · 1989 · $243,673

## Abstract

The aim of this proposal is to identify cDNA clones corresponding
to genes preferentially expressed in CD3- NK cells and other cells
that express NK function and to understand the function of the
corresponding gene products.  A subtracted cDNA library enriched
for cDNA clones associated with NK cells and NK/LAK activity will
be prepared from a cloned NK cell (CD3-,CD16+,Leu19+) that
expresses high-level NK/LAK function.  A variety of screening
methods will identify cDNA clones of interest within  this library.
Our first priority is to identify cDNA clones encoding cell surface
molecules associated with NK cells and/or NK and LAK function.
Second, we are interested in identifying clones encoding cell
surface molecules expressed in both NK and T cells; this may
identify molecules important in the regulation of NK function.
Third, we are interested in clones that encode other molecules
expressed preferentially in NK cells and other cells that express
NK function.  Two types of probes will be used to identify cDNA
clones that encode membrane-bound components or secreted products.
First, the subtracted library will be screened with a probe derived
from poly-A+ RNA from membrane-bound polysomes of the cloned NK
cell.  Second, antisera prepared against the NK cell clone and
absorbed with LCL will be used to probe the subtracted cDNA library
prepared in a  lambda-based expression vector.  A long-term goal
of this project is to use information and reagents obtained by
molecular techniques to understand further the cell biology of NK
cells, especially the generation and expression of NK/LAK activity.
Whereas all genes to be studied will be derived from the NK clone
described above, probing for expression of these genes in other
cells, e.g.  CD3+ cells with NK or NK and LAK function may help
understand the genetic basis of these functions.  Newly-defined
genes will be sequenced and their expression studied in a variety
of cell types; antisera/moAb will be made to proteins of interest.
Although more difficult, we also plan to perform anti-sense (anti-
mRNA) inhibition and transfection studies.  We anticipate that
these studies will provide information that will be useful as
activated NK cells are used in clinical protocols.

## Key facts

- **NIH application ID:** 3128420
- **Project number:** 2R01AI019007-06A1
- **Recipient organization:** UNIVERSITY OF MINNESOTA
- **SAM.gov UEI:** KABJZBBJ4B54
- **Principal Investigator:** FRITZ H BACH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $243,673
- **Award type:** 2
- **Project period:** 1983-05-01 → 1993-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128420

## Citation

> US National Institutes of Health, RePORTER application 3128420, MOLECULAR STUDIES OF NK CELLS AND NK/LAK FUNCTION (2R01AI019007-06A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128420. Licensed CC0.

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