# FINE STRUCTURE ANALYSIS OF HLA-D

> **NIH NIH R01** · UNIVERSITY OF MINNESOTA · 1987 · $268,118

## Abstract

The present grant proposes to use lymphoblastoid cell line (LCL) variants
that have lost expression of different portions of the HLA-D region (of one
or both D haplotypes) to permit both genetic mapping of HLA-D encoded
determinants and studies of genetic complementation.  Preliminary
investigations have demonstrated the feasibility of creating loss variants
of varying lengths of the HLA-D region (including DNA loss variants) and
mapping of determinants within this region, using cloned primed lymphocyte
typing (PLT) reagents.  Such reagents are of key import both because they
offer a highly productive approach for the definition of multiple
determinants associated with single D haplotypes, and because they can be
readily prepared against normal cells (or LCLs) in any given combination.
We shall focus first on DR2+ and DR4+ haplotypes, since these antigens are
found in association with a large number of different "Dw antigenic
clusters", defined with homozygous typing cells and PLT reagents.  DNA
probes will be used to determine the nature of the mutation underlying loss
of antigen expression and to aid in construction of a genetic map of the D
region, as well as to further our understanding of the genetic basis for
the relationship between serologically and cellularly detected determinants
of D region encoded molecules.  Attempts will be made to generate
serological reagents, including monoclonal antibodies against D region
products.  Molecules expressing DR determinants (and later other D region
encoded molecules expressing serologically detected determinants) will be
isolated and studied by peptide mapping to investigate whether some
peptides are associated with the serologically detectable determinant and
others with the cellularly defined determinant(s) within a single molecular
entity.  Finally, we shall perform studies on a molecule, B7/21, which is
encoded by genes linked to HLA-DR but which is different from the presently
defined D region encoded molecules.  We expect that these various
approaches will contribute to the generation of a coherent picture of the
genetic structure of, and molecules encoded in, the D region and thus
enhance continued progress in matching for transplantation and
understanding of disease associations.

## Key facts

- **NIH application ID:** 3128423
- **Project number:** 5R01AI019007-05
- **Recipient organization:** UNIVERSITY OF MINNESOTA
- **SAM.gov UEI:** KABJZBBJ4B54
- **Principal Investigator:** FRITZ H BACH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $268,118
- **Award type:** 5
- **Project period:** 1983-05-01 → 1988-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128423

## Citation

> US National Institutes of Health, RePORTER application 3128423, FINE STRUCTURE ANALYSIS OF HLA-D (5R01AI019007-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128423. Licensed CC0.

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