# INHERITED CONTROL MECHANISMS IN IG GENE EXPRESSION

> **NIH NIH R01** · MOUNT SINAI SCHOOL OF MEDICINE OF CUNY · 1989 · $192,713

## Abstract

The broad objective of this research is to investigate T cell
responses to self and nonself immunoglobulins.  The fact that IgG2a
antigens are naturally expressed in Ia B lymphoma cells known to
be fully capable of antigen presentation and that the structure of
the antigen is readily manipulated using recombinant DNA techniques
gives this system unique advantages for studying possible
relationships between complex intracellular trafficking pathways
and MHC restriction specificity.  We plan to construct pSV2gt
vectors encoding various forms of the C57BL/6 IgG2ab CH3.  We are
particularly interested in this portion of the molecule because
allotypic determinants recognized by Igh-1b-specific T cell are
located in the CH3 domain.  Soluble, membrane-bound,and cytoplasmic
form(s) of the C57BL/6 IgG2ab CH3 will be expressed in transfected
B lymphoma cell lines.  We will characterize the protein products
that are synthesized and test whether these molecules are presented
to Class II-restricted T cells.  A major goal is to determine
whether endogenously synthesized IgG2a antigens become associated
with Class II molecules intracellularly.  We will also express the
VMPC11 -C57BL/6 IgG2ab CH3 protein under control of the human B-
actin promoter in a variety of different types of Ia+ APC lines to
test whether endogenous IgG2a synthesized by different types of Ia+
APC will stimulate Igh-1b-specific T cells.  Overall, these
experiments will hopefully lead to a clearer understanding of the
possible role of antigen-presenting cells in MHC-restricted T cell
recognition of self immunoglobulins and processes involved in T
cell tolerance.

## Key facts

- **NIH application ID:** 3128483
- **Project number:** 2R01AI019047-07A1
- **Recipient organization:** MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
- **Principal Investigator:** ELIZABETH K BIKOFF
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $192,713
- **Award type:** 2
- **Project period:** 1981-09-01 → 1994-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128483

## Citation

> US National Institutes of Health, RePORTER application 3128483, INHERITED CONTROL MECHANISMS IN IG GENE EXPRESSION (2R01AI019047-07A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128483. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*