# INHERITED CONTROL MECHANISMS IN IG GENE EXPRESSION

> **NIH NIH R01** · MOUNT SINAI SCHOOL OF MEDICINE OF CUNY · 1987 · $160,406

## Abstract

The broad objective of this research is to provide new information on the
mechanism of Lyl+ T cell tolerance for self Ig.  We recently developed an
immunoglobulin-dependent T cell proliferation assay that measures Lyl+ T
cell reactivity to self and nonself Ig.  Using allotype congenic mice, we
demonstrated that responding Lyl+ T cells recognize allotypic determinants
present on the foreign Ig and that this response is sensitive to tolerance
induction.  The fact that the antigen is well characterized structurally,
easily quantitated by standard techniques, naturally present in serum and
under normal physiological conditions also exists as an integral membrane
glycoprotein, as well as the availability of a well defined genetic
polymorphism and allotype congenic mouse strains, provide this system with
unique advantages for studying the cellular basis of tolerance.  The major
goal of the proposed investigation is to identify the types of cells that
participate in tolerance induction.  Adult thymectomized mice,
radiation-induced bone marrow chimeras, experimentally agammaglobulinemic,
and allotype-suppressed mice will be used as tools to characterize the
target population and further define the role played by B cells.  The
possibility that Ly2+ suppressor T cells may be involved in the induction
and/or maintenance of Lyl+ T cell tolerance will be tested.  Specific
structural features of the IgG2a molecule required for tolerance induction
will be described.  Moreover, a wide panel of myeloma and hybridoma
proteins, including mutant monoclonal antibodies with well characterized
structural alterations are available for analyzing the possible
relationship between immunogenic and tolerogenic determinants.  In sum,
these studies will hopefully lead to a clearer understanding of cellular
events in tolerance and processes involved in immunoglobulin homeostasis.

## Key facts

- **NIH application ID:** 3128485
- **Project number:** 5R01AI019047-06
- **Recipient organization:** MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
- **Principal Investigator:** ELIZABETH K BIKOFF
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $160,406
- **Award type:** 5
- **Project period:** 1981-09-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128485

## Citation

> US National Institutes of Health, RePORTER application 3128485, INHERITED CONTROL MECHANISMS IN IG GENE EXPRESSION (5R01AI019047-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128485. Licensed CC0.

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