# HUMAN LEUKOCYTE SUBPOPULATIONS

> **NIH NIH R01** · DARTMOUTH COLLEGE · 1987 · $172,530

## Abstract

Immunoglobulins of the IgA class are important in the specific immune
defense of mucosal surfaces and perhaps in the regulation of secretory
immunity.  However, the mechanicsms involved in these activities are still
incompletely understood.  The recent demonstration that lymphocytes and
effector cells populations display receptors for the Fc portion of IgA
(RFc), suggests that these receptors may mediate interactions between IgA
and/or IgA-antigen complexes and the effector or regulatory cell expressing
RFc.  The present proposal is directed toward an evaluation of the function
of RFc and RFc - bearing human leukocyte subpopulations.  We would first
quantitate the expression of this receptor on various lymphocyte
subpopulations and on leukocytes from peripheral blood and mucosal
surfaces.  Since the quantity of RFc per cell may be related to its
functional activity, we would examine the ability of various mediators of
immune function including supernatants from stimulated lymphocytes,
immunoglobulins and immunoglobulin-antigen complexes to modulate expression
of RFc on these cells.  RFc would be isolated and partially characterized.
Monoclonal antibodies to RFc would be prepared and used for further
characterization of these receptors, and in studies of their function.  The
regulatory and effector properties of cells bearing RFc would be examined
and the role of RFc in these functions would be determined.  These studies
would be performed on cells from peripheral and mucosal lymphoid tissues
before and after modulation of RFc expression.  In addition, myeloid
cell-specific monoclonal antibodies would be used to isolate subpopulations
of monocytes and PMNs for the subsequent analysis of the ability of these
groups of cells to manifest RFc -dependent effector functions.  The data
obtained in these studies on RFc and RFc -bearing leukocyte subpopulations
should enhance our understanding of the regulation and expression of
secretory immunity.

## Key facts

- **NIH application ID:** 3128495
- **Project number:** 5R01AI019053-07
- **Recipient organization:** DARTMOUTH COLLEGE
- **SAM.gov UEI:** EB8ASJBCFER9
- **Principal Investigator:** MICHAEL W FANGER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $172,530
- **Award type:** 5
- **Project period:** 1981-09-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128495

## Citation

> US National Institutes of Health, RePORTER application 3128495, HUMAN LEUKOCYTE SUBPOPULATIONS (5R01AI019053-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128495. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*