# HUMAN LEUKOCYTE SUBPOPULATIONS

> **NIH NIH R01** · DARTMOUTH COLLEGE · 1990 · $226,413

## Abstract

During the past several years it has become clear that there are
three distinct Fc receptors for IgG (Fc gamma R) which are
associated with human myeloid cells and thus potentially involved
in antibody dependent killing (ADK) by these cells.  With the
development of monoclonal antibodies (MAb) to each of these Fc
gamma R, it has now become possible to explore in a definitive
way the properties and functional capabilities of each receptor.
We have been involved in the development of MAb to these Fc
gamma R which have been used by us and by other investigators
to develop a substantial base of information on the functional
properties of each of these receptors.  We now propose to use
these MAb and the hybridoma cells that produce them to
systematically explore the functional properties of the different
Fc gamma R.  In particular, we propose to use Ig bearing
hybridoma cells as targets to further define the cytotoxic
potential of different effector cells and trigger molecules as well
as to develop insights into the triggering events, their
requirements and mechanisms.  To facilitate our studies on one of
these receptors, Fc gamma R II, we would develop a panel of MAb
to Fc gamma RII comparable to those we have already prepared
against Fc gamma RI.  MAb which react with Fc gamma RII at
epitopes other than the ligand binding site would be used in an
analysis of the initial events associated with ADK through this
receptor.  Studies would be carried out on the ability of MAb
specific for different Fc gamma R to modulate the expression of
these receptors.  The conditions for, and effects of, triggering of
each of the Fc gamma R on different effector cells would be
examined.  This analysis would include studies of early signaling,
superoxide generation, enzyme and monokine release and
phagocytosis.  The ability of various biological mediators to
modulate the expression and cytotoxic capabilities of each of the
Fc gamma R and the effector cells with which they are associated
would also be determined.  Finally, we would prepare and use
heteroantibodies for dissection of the functional activities of the
different Fc gamma R.  Specifically, we would investigate the
capability of each Fc gamma R and their associated effector cells
to mediate killing and/or phagocytosis of red blood cell, bacterial,
parasite and nucleated cell targets.  These studies should permit
us to clearly define the functional properties of each of the Fc
gamma R, their capability to mediate killing of a variety of
target cells, and their associated cytotoxic mechanisms.

## Key facts

- **NIH application ID:** 3128497
- **Project number:** 5R01AI019053-10
- **Recipient organization:** DARTMOUTH COLLEGE
- **SAM.gov UEI:** EB8ASJBCFER9
- **Principal Investigator:** MICHAEL W FANGER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $226,413
- **Award type:** 5
- **Project period:** 1981-09-01 → 1993-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128497

## Citation

> US National Institutes of Health, RePORTER application 3128497, HUMAN LEUKOCYTE SUBPOPULATIONS (5R01AI019053-10). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3128497. Licensed CC0.

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