# ACTIVE TRANSPORT OF MALTOSE IN ESCHERICHIA COLI

> **NIH NIH R01** · COLUMBIA UNIV NEW YORK MORNINGSIDE · 1987 · $152,263

## Abstract

The membranes of all living cells contain specific transport proteins that
enable the cell to accumulate nutrients and to maintain appropriate
concentration gradients of ions.  We will study the structure and function
of a specific active transport system in order to gain some understanding
of how this process occurs.

The transport system that will be studied is the maltose/maltodextrin
transport system of Escherichia coli.  This system is composed of five
protein subunits which enable the cell to actively pump maltose and longer
polymers of glucose (maltodextrins) into the cytoplasm.

We will use biochemical techniques to localize the sites that interact with
substrate molecules.  In addition we will use specific labels for locating
the site(s) at which the energy donor interacts with the system.  Finally
we will define the location at which the water soluble maltose binding
protein interacts with the membrane bound components of the system.

We will also use a genetic approach to complement these biochemical
experiments.  We will isolate mutants with altered substrate specificity to
localize the substrate recognition site genetically.  In addition we will
isolate mutants in which the interaction of the membrane proteins with
maltose binding protein has been altered.  We will determine the sites of
these mutations by DNA sequencing and comparing the mutant sequence with
the known wild-type sequence.

Our long term goal is to generate a picture of how substrate molecules
interact with the proteins during the process of translocation through the
membrane and how this process is coupled to energy.

## Key facts

- **NIH application ID:** 3128631
- **Project number:** 5R01AI019276-06
- **Recipient organization:** COLUMBIA UNIV NEW YORK MORNINGSIDE
- **SAM.gov UEI:** F4N1QNPB95M4
- **Principal Investigator:** HOWARD A SHUMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $152,263
- **Award type:** 5
- **Project period:** 1982-08-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128631

## Citation

> US National Institutes of Health, RePORTER application 3128631, ACTIVE TRANSPORT OF MALTOSE IN ESCHERICHIA COLI (5R01AI019276-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128631. Licensed CC0.

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