# NATURE OF INDUCER AND SUPPRESSOR

> **NIH NIH R01** · NEW YORK UNIVERSITY · 1987 · $167,435

## Abstract

Our objectives are to exploit our discovery, isolation and partial
characterization of two opposing antigen-specific immunoregulatory
activities found in T-lymphocyte dialyates:  an Inducer Factor (TiF) which
augments cell-mediated immunity (CMI) and a Suppressor Factor (TsF) which
suppresses CMI in immune cell populations and neutralizes the activity of
Inducer Factor on nonimmune cell populations.

Specific aims are centered around the logical progress toward production of
recombinant Inducer Factor in E. coli, once the amino acid sequences of
purified Bovine Inducer Factor and Human Inducer Factor are determined.

Studies include:  a) continuation of amino acid analyses and beginning
amino acid sequencing of nanomole quantities of Diphtheria toxoid specific
and Tetanus toxoid specific Bovine Inducer Factors purified by binding to
specific antigen and hydrolyzed following recovery from the respective
solid-phase immunoadsorbant; b) we plan to continue studies on the
production and assay of TiF by hybridoma clones of Leukemia TH cells fused
to normal antigen-stimulated TH cells of various antigenic specificities.
Knowledge of the amino acid composition and sequence of Bovine Inducer
Factor will allow the choice of an appropriate radiolabel to internally
radiolabel the hybridoma clones now producing TiF and permit isolation of
labelled TiF by specific solid-phase antigen immunoadsorbants and recovery
following treatment with 8M urea for amino acid analyses and sequencing; c)
similar studies will be done on TiF generated from antigen-stimulated
normal TH cells propagated by IL-2 in continuous culture.  Additionally,
antiidiotypic (antiparatopic) T-cells will be generated in such cultures
and their dialysates studied for TiF and TsF activity in the LMI assay in
vitro and BALB/c mice footpad reaction in vivo.

It is also planned to extend studies to clarify the relationship of TiF to
the T-cell receptor for antigen by absorption of dialysates with antibodies
directed at putative T-cell receptor molecules.  Similar studies are
planned to clarify the relationship of TiF to V-region structures using a
variety of monoclonal anti-VH antibodies.

The Leukocyte Migration Inhibition (LMI) assay will be used as a screening
test in the above studies preparatory to and guiding the design of in vivo
studies of effects of TiF and TsF and the DTH footpad reaction in BALB/c
mice.

## Key facts

- **NIH application ID:** 3128756
- **Project number:** 5R01AI019369-05
- **Recipient organization:** NEW YORK UNIVERSITY
- **SAM.gov UEI:** NX9PXMKW5KW8
- **Principal Investigator:** SHERWOOD A LAWRENCE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $167,435
- **Award type:** 5
- **Project period:** 1982-09-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128756

## Citation

> US National Institutes of Health, RePORTER application 3128756, NATURE OF INDUCER AND SUPPRESSOR (5R01AI019369-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128756. Licensed CC0.

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