# LOCATION OF BACTERIAL PROLIFERATION IN SALMONELLOSIS

> **NIH NIH R01** · VIRGINIA COMMONWEALTH UNIVERSITY · 1988 · $77,318

## Abstract

Murine salmonellosis is used as the experimental model to resolve the
current controversy of whether pathogenic salmonellae do survive and
multiply within host phagocytes.  Electron microscopic studies on the
peritoneal exudate cells and on the liver of mice infected with Salmonella
typhimurium have now shown that salmonellae are killed within inflammatory
polymorphs and macrophages and multiply in the extracellular space and
within hepatocytes.

Continuation of this project in the next three years will be directed (a)
to certify that the bacteria seen in the tissues are in fact salmonellae
and that intracytoplasmic debris seen in the phagocytes does contain
salmonella antigens, using immunocytochemical techniques (such as
immunofluorescent staining, glucose oxidase and colloidal gold labeling) in
light and electron microscopy; (b) to develop a tissue embedding method so
that serial sections can be made for histopathological and
immunocytochemical staining for use both in light and electron microscopy;
(c) to determine whether there is any difference in the preferential
location of bacterial proliferation in host tissues and, in particular, the
role of the macrophages in salmonellosis, by comparing genetically
resistant versus susceptible mice infected with salmonellae.

The project is considered as a crucial step in the elucidation of
pathogenesis of and immunity in murine salmonellosis, particularly in view
of the current interest in the genetic variations of host response to
infection.  Such a definitive study is essential in providing the basis for
the development of salmonella vaccines.

## Key facts

- **NIH application ID:** 3128791
- **Project number:** 5R01AI019434-04
- **Recipient organization:** VIRGINIA COMMONWEALTH UNIVERSITY
- **SAM.gov UEI:** MLQFL4JSSAA9
- **Principal Investigator:** HSIU-SHENG HSU
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $77,318
- **Award type:** 5
- **Project period:** 1984-09-30 → 1989-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128791

## Citation

> US National Institutes of Health, RePORTER application 3128791, LOCATION OF BACTERIAL PROLIFERATION IN SALMONELLOSIS (5R01AI019434-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128791. Licensed CC0.

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