# PROLIFERATION AND FUNCTION OF MONONUCLEAR PHAGOCYTES

> **NIH NIH R01** · ROSWELL PARK CANCER INSTITUTE · 1990 · $199,453

## Abstract

The object of this project is to define the genetic basis for the
regulation of mononuclear phagocyte proliferation, differentiation
and functional heterogeneity.  In addition, we wish to define the
role hematopoietic growth factors play in the control of their
production from the multipotent hematopoietic stem cell.  We
will approach this objective by using currently available
antibodies to differentiation antigens to identify and sort, using
flow cytometry, cells at unique differentiation stages.  By
culturing these sorted cells in the presence of the selected
hematopoietic growth factors IL3, GM-CSF and M-CSF, we will
determine their differentiation kinetics.  This will be
accomplished by using a panel or monoclonal antibodies to define
the phenotype of the progeny which emerge as a function of time
from the sorted precursors.

We will also investigate the expression of proto-oncogene
products using currently available antibodies.  This expression will
be correlated with the proliferation stages of the cells as well as
their differentiation stage.  In this way, we will be able to
determine if the gene's expression is cell cycle related and
independent of differentiation, whether its expression is related
to differentiation or both.

Results of these studies will have an enormous impact on our
understanding of how a normal cell's production and function is
controlled in health and disease.

## Key facts

- **NIH application ID:** 3128834
- **Project number:** 5R01AI019490-09
- **Recipient organization:** ROSWELL PARK CANCER INSTITUTE
- **Principal Investigator:** CARLETON C STEWART
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $199,453
- **Award type:** 5
- **Project period:** 1988-07-01 → 1992-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128834

## Citation

> US National Institutes of Health, RePORTER application 3128834, PROLIFERATION AND FUNCTION OF MONONUCLEAR PHAGOCYTES (5R01AI019490-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128834. Licensed CC0.

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