# REGULATORY MECHANISMS OF MACROPHAGE ACTION

> **NIH NIH R01** · UNIVERSITY OF MARYLAND BALTIMORE · 1988 · $183,370

## Abstract

Studies with erythrocytes and artificial lipid bilayers in many
laboratories have yielded the fundamental features of membrane
attack mechanisms by complement (C).  Consequently, our
research during the last few years has been devoted to
understanding the mechanism(s) of C-mediated cell death in
metabolically active nucleated cells (NC).  We have found that
cytolysis of NC requires multiple C5b-9 channels, due to the
defense mechanism of the cell to survive limited C attack by
eliminating the potentially lytic C channels.  In addition, the
channels, when present in sublytic numbers, cause a transient
increase in cellular Ca2+ and exert a variety of biological effects
such as activation of phospholipases, lipid methyltransferases, and
neutral proteases in the cell.  Our program is aimed at studying
the mechanisms by which C5b-9 mediates cell death, stimulates
the cell to repair limited injury, and modulates cellular functions
that are relevant to the pathophysiology of the target cells in
inflammation and immunity.

Specifically, we want to determine the C-induced biochemical
signals that stimulate the repair process.  We will also investigate
whether  cell death elicited by the remaining channels is entirely
dependent on colloid-osmotic deregulation, and if applicable
which metabolic pathways are involved in the process of cell
death.  In addition, we will examine whether metabolic
stimulation of repair processes similar to those produced by C5b-9
also occur in targets following limited attack by channel-forming
proteins derived from CTL or NK-cells.  We will continue to study
the structure and functional aspects of C8-binding protein, and its
role in nucleated cell killing and stimulation by homologous C5b-
9.

These studies, collectively, will significantly increase our
knowledge on the biological activities of complement channels
and other channel forming immune effectors on NC targets.

## Key facts

- **NIH application ID:** 3128946
- **Project number:** 2R01AI019622-06
- **Recipient organization:** UNIVERSITY OF MARYLAND BALTIMORE
- **SAM.gov UEI:** Z9CRZKD42ZT1
- **Principal Investigator:** MOON L SHIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $183,370
- **Award type:** 2
- **Project period:** 1983-01-01 → 1992-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128946

## Citation

> US National Institutes of Health, RePORTER application 3128946, REGULATORY MECHANISMS OF MACROPHAGE ACTION (2R01AI019622-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128946. Licensed CC0.

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