# TRANSPLANTATION ANTIGENS - EXPRESSION AND FUNCTION

> **NIH NIH R01** · CALIFORNIA INSTITUTE OF TECHNOLOGY · 1987 · $193,604

## Abstract

We propose to study the nature of the T-cell recognition structures
visualized by allogeneic killer T cells and virus-restricted killer T
cells.  We will use in vitro mutagenesis to delete, shuffle, and mutate the
exons encoding a classical transplantation antigen, the Ld molecule.  These
mutant forms of the Ld gene will then be transferred into mouse L cells,
teratocarcinoma cells, and hopefully into normal bone marrow cells.  The
ability of the mutated Ld molecule to mediate T-cell killing, to interact
with B2-microglobulin, and to interact with virus antigens will then be
assessed to shed insights into the natures of the interactions among these
molecules in the T-cell recognition structure.  We also plan to study the
DNA elements which control the expression of several class I genes in
various cell lines and, if possible, in mice.  Again the class I genes will
be altered by in vitro mutagenesis and their expression assessed after
appropriate gene transfer experiments.

## Key facts

- **NIH application ID:** 3128959
- **Project number:** 5R01AI019624-05
- **Recipient organization:** CALIFORNIA INSTITUTE OF TECHNOLOGY
- **SAM.gov UEI:** U2JMKHNS5TG4
- **Principal Investigator:** LEROY E HOOD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $193,604
- **Award type:** 5
- **Project period:** 1983-01-01 → 1987-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128959

## Citation

> US National Institutes of Health, RePORTER application 3128959, TRANSPLANTATION ANTIGENS - EXPRESSION AND FUNCTION (5R01AI019624-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128959. Licensed CC0.

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